ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAEFSADLCL FDLDGTIVST TVAAEKAWTK LCYEYGVDPS ELFKHSHGAR TQEVLRRFFP 

        70         80         90        100        110        120 
KLDDTDNKGV LALEKDIAHS YLDTVSLIPG AENLLLSLDV DTETQKKLPE RKWAIVTSGS 

       130        140        150        160        170        180 
PYLAFSWFET ILKNVGKPKV FITGFDVKNG KPDPEGYSRA RDLLRQDLQL TGKQDLKYVV 

       190        200        210        220        230        240 
FEDAPVGIKA GKAMGAITVG ITSSYDKSVL FDAGADYVVC DLTQVSVVKN NENGIVIQVN 


NPLTRA 

References and documentation are available.

Number of amino acids: 246

Molecular weight: 27099.88

Theoretical pI: 5.19

Amino acid composition: 
Ala (A)  19	  7.7%
Arg (R)   8	  3.3%
Asn (N)   9	  3.7%
Asp (D)  21	  8.5%
Cys (C)   3	  1.2%
Gln (Q)   7	  2.8%
Glu (E)  13	  5.3%
Gly (G)  17	  6.9%
His (H)   3	  1.2%
Ile (I)  11	  4.5%
Leu (L)  26	 10.6%
Lys (K)  20	  8.1%
Met (M)   2	  0.8%
Phe (F)  11	  4.5%
Pro (P)  10	  4.1%
Ser (S)  15	  6.1%
Thr (T)  17	  6.9%
Trp (W)   3	  1.2%
Tyr (Y)   8	  3.3%
Val (V)  23	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1220
Hydrogen    H	      1923
Nitrogen    N	       315
Oxygen      O	       371
Sulfur      S	         5

Formula: C1220H1923N315O371S5
Total number of atoms: 3834

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28545
Abs 0.1% (=1 g/l)   1.053, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   1.049, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 14.74
This classifies the protein as stable.



Aliphatic index: 93.50

Grand average of hydropathicity (GRAVY): -0.151