ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAEINKQSNK WEQEEVSNEN NWELAEEESV DWRGRPSNPN KHGGMRAALF VLGLQAFEIM 

        70         80         90        100        110        120 
GIAAVGNNLI TYVINEMHFP LSKAANIVTN FVGTIFIFAL LGGYLSDAFL GSFWTIIIFG 

       130        140        150        160        170        180 
FVELSGFILL SVQAHLPQLK PPKCNPLIDQ TCEEAKGFKA MIFFMALYLV ALGSGCVKPN 

       190        200        210        220        230        240 
MIAHGADQFS QSHPKQSKRL SSYFNAAYFA FSMGELIALT LLVWVQTHSG MDIGFGVSAA 

       250        260        270        280        290        300 
AMTMGIISLV SGTMYFRNKR PRRSIFTPIA HVIVAAILKR KLASPSDPRM LHGDHHVAND 

       310        320        330        340        350        360 
VVPSSTLPHT PRFRFLDKAC IKIQDTNTKE SPWRLCTVTQ VEQVKTLISL VPIFASTIVF 

       370        380        390        400        410        420 
NTILAQLQTF SVQQGSSMNT RLSNSFHIPP ASLQAIPYIM LIFLVPLYDS FLVPFARKLT 

       430        440        450        460        470        480 
GHNSGIPPLT RIGIGLFLST FSMVSAAMLE KKRRDSSVLD GRILSIFWIT PQFLIFGISE 

       490        500        510        520        530        540 
MFTAVGLIEF FYKQSAKGME SFLMALTYCS YSFGFYFSSV LVSVVNKITS TSVDSKGWLG 

       550        560        570        580        590 
ENDLNKDRLD LFYWLLAVLS LLNFLSYLFW SRWNIKSSRR NNTNVVGDEN I 

References and documentation are available.

Number of amino acids: 591

Molecular weight: 66041.83

Theoretical pI: 9.10

Amino acid composition: 
Ala (A)  42	  7.1%
Arg (R)  23	  3.9%
Asn (N)  31	  5.2%
Asp (D)  18	  3.0%
Cys (C)   6	  1.0%
Gln (Q)  20	  3.4%
Glu (E)  23	  3.9%
Gly (G)  36	  6.1%
His (H)  13	  2.2%
Ile (I)  45	  7.6%
Leu (L)  64	 10.8%
Lys (K)  27	  4.6%
Met (M)  20	  3.4%
Phe (F)  45	  7.6%
Pro (P)  26	  4.4%
Ser (S)  58	  9.8%
Thr (T)  30	  5.1%
Trp (W)  11	  1.9%
Tyr (Y)  14	  2.4%
Val (V)  39	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      3030
Hydrogen    H	      4691
Nitrogen    N	       775
Oxygen      O	       827
Sulfur      S	        26

Formula: C3030H4691N775O827S26
Total number of atoms: 9349

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81735
Abs 0.1% (=1 g/l)   1.238, assuming all pairs of Cys residues form cystines


Ext. coefficient    81360
Abs 0.1% (=1 g/l)   1.232, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.12
This classifies the protein as unstable.



Aliphatic index: 98.17

Grand average of hydropathicity (GRAVY): 0.237