ProtParam
User-provided sequence:
10 20 30 40 50 60
MAEISHPPME QLQDLEYCID SNPPWPETVL LAFQNYILML GTSAFIPALL VPAMGGSDGD
70 80 90 100 110 120
RARVIQTLLF VAGIKTLLQA LFGTRLPAVV GGSLAYVVPI AYIINDSSLQ KISNDHERFI
130 140 150 160 170 180
HTMRAIQGAL IVASSIQIIL GYSQVWGLFS RFFSPLGMAP VVGLVGLGMF QRGFPQLGNC
190 200 210 220 230 240
IEIGLPMLLL VIGLTQYLKH VRPFKDVPIF ERFPILICVT IVWIYAVILT ASGAYRGKPS
250 260 270 280 290 300
LTQHSCRTDK ANLISTAPWF KFPYPLQWGP PTFSVGHSFA MMSAVLVSMV ESTGAYIAAS
310 320 330 340 350 360
RLAIATPPPA YVLSRGIGWQ GIGVLLDGLF GTGTGSTVLV ENVGLLGLTR VGSRRVVQVS
370 380 390 400 410 420
AGFMIVFSTL GKFGAVFASI PVPIYAALHC ILFGLVAAVG LSFLQFTNMN SMRNLMITGL
430 440 450 460 470 480
SLFLGISIPQ FFAQYWDARH YGLVHTNAGW FNAFLNTLFM SPATVGLIIA VFMDNTMEVE
490 500 510 520
RSKKDRGMPW WVKFRTFRGD NRNEEFYTLP FNLNRFFPPT
References and
documentation are available.
Number of amino acids: 520
Molecular weight: 57131.20
Theoretical pI: 9.33
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 2652
Hydrogen H 4096
Nitrogen N 668
Oxygen O 689
Sulfur S 24
Formula: C
2652H
4096N
668O
689S
24
Total number of atoms: 8129
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 77600
Abs 0.1% (=1 g/l) 1.358, assuming all pairs of Cys residues form cystines
Ext. coefficient 77350
Abs 0.1% (=1 g/l) 1.354, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.40
This classifies the protein as stable.
Aliphatic index: 105.56
Grand average of hydropathicity (GRAVY): 0.500