ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAEKILENKD PKLEAMTVDH EVSAPKPIPV DEPTLTRVAR PLRHVRHIPV KSLVFHSKHG 

        70         80         90        100        110        120 
PITFSYENKI KLPISKNKLV VQVNYVGLNP VDMKIRNGYT KPIYGEAGIG REYSGVITHV 

       130        140        150        160        170        180 
GDNLTNRWNV GDDVYGIYYH PKLAIGALQS SLLIDPRVDP ILMRPKNTLS PEKAAGSLFC 

       190        200        210        220        230        240 
LGTALNLLAQ LKEKDQLNTE SNVLINGGTS SVGMFAIQLL KRYYKVSKKL VVVTSGNGAA 

       250        260        270        280        290        300 
VLSEHFPDLK DEIIFINYLS CRGKSSKPLR RMLDTGKVVD YDDFNTLKET EDYTQGKFNV 

       310        320        330        340        350        360 
VLDFIGGYDI LSHSSSLIHA KGAYITTVGD YVGNYKKDVF DSWDNPSANA RKMFGSMLWS 

       370        380        390        400        410        420 
YDYSHFYFDP NIKIIPKKND WIHECGKLLN EGVVDCVVDK VYSWKNFKEA FSYMATQRAQ 

       430 
GKLIMKVEGF 

References and documentation are available.

Number of amino acids: 430

Molecular weight: 48370.69

Theoretical pI: 8.95

Amino acid composition: 
Ala (A)  21	  4.9%
Arg (R)  15	  3.5%
Asn (N)  25	  5.8%
Asp (D)  28	  6.5%
Cys (C)   4	  0.9%
Gln (Q)   8	  1.9%
Glu (E)  19	  4.4%
Gly (G)  31	  7.2%
His (H)  12	  2.8%
Ile (I)  28	  6.5%
Leu (L)  39	  9.1%
Lys (K)  40	  9.3%
Met (M)  10	  2.3%
Phe (F)  16	  3.7%
Pro (P)  21	  4.9%
Ser (S)  30	  7.0%
Thr (T)  19	  4.4%
Trp (W)   5	  1.2%
Tyr (Y)  22	  5.1%
Val (V)  37	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2191
Hydrogen    H	      3433
Nitrogen    N	       577
Oxygen      O	       629
Sulfur      S	        14

Formula: C2191H3433N577O629S14
Total number of atoms: 6844

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60530
Abs 0.1% (=1 g/l)   1.251, assuming all pairs of Cys residues form cystines


Ext. coefficient    60280
Abs 0.1% (=1 g/l)   1.246, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.11
This classifies the protein as stable.



Aliphatic index: 90.60

Grand average of hydropathicity (GRAVY): -0.272