ProtParam
User-provided sequence:
10 20 30 40 50 60
MAELNDYSTM IDILLSDMDL ETVTTKKVRM ALKEVYAIDV ESQGKAINKL IRKHLDLVKE
70 80 90 100 110 120
RPRFERSLED LLKENATLAI ELTKEITVSK RSSGEEKNDS ETKGTHVEKK KGTVSKSPIS
130 140 150 160 170 180
TRKVTLSKSL ASLLGEHELT RTEVVRRLWA YIKAHNLQNP NNKKEILCDE KLELILGKST
190 200 210 220
NMFEMHKILA SHMTEPKKIS DCPPLIQEVR RKEKPIVSDS EQSDTKGI
References and
documentation are available.
Number of amino acids: 228
Molecular weight: 25967.03
Theoretical pI: 8.86
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1132
Hydrogen H 1903
Nitrogen N 319
Oxygen O 356
Sulfur S 9
Formula: C
1132H
1903N
319O
356S
9
Total number of atoms: 3719
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 10095
Abs 0.1% (=1 g/l) 0.389, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 0.384, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.00
This classifies the protein as unstable.
Aliphatic index: 92.76
Grand average of hydropathicity (GRAVY): -0.617