ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAEPEVIHSW SAPRSLSTSL MYSFAQRLDT EVVDEPLYAS FLKATGFDRP YRDEVLSKME 

        70         80         90        100        110        120 
CNGEKVVKDV IYGSGSKKYR YCKHISKQRL FGLPSELMSR GKHFILIRNP LNILPSFEKV 

       130        140        150        160        170        180 
HPPSFLELGL GELVSIYSDL CQMGTPPAVI DADELQRDPE TTLRGLCDDL EIPFQASMLK 

       190        200        210        220        230        240 
WKAGPIPEDG VWAPWWYKSV HESTGFSSPK KYPRTFPLSH YDLLERSLPL YNILRSHVKH 

       250        260        270        280        290        300 
SSSLLSSPLP PPSLPVPENA KLLAWVGDEI LPREMAKVSV FDSVVQGGDS VWEGLRIYKG 

       310        320        330        340        350        360 
KIFKLEEHLD RLFDSAKALA FDNVPAREEV KEAIFRTLIT NGMFDNTHIR LSLTRGKKVT 

       370        380        390        400        410        420 
SGMSPAYNRY GCTLIVLAEW KPPVYDNEGG IVLVTATTRR NSPNNLDSKI HHNNLLNNIL 

       430        440        450        460        470        480 
AKIESNNTNA ADAIMLDKDG YVSETNATNI FMVKKGCVLT PHADYCLPGI TRATVMELVV 

       490        500        510        520        530        540 
KENFILEERR ISLSEFHTAN EVWTTGTMGE LSPVVKIDGR VIGDGKVGPV TRTLQNAYKK 

       550 
LTEDSGVPIP TYQEP 

References and documentation are available.

Number of amino acids: 555

Molecular weight: 62212.34

Theoretical pI: 6.42

Amino acid composition: 
Ala (A)  30	  5.4%
Arg (R)  28	  5.0%
Asn (N)  25	  4.5%
Asp (D)  29	  5.2%
Cys (C)   7	  1.3%
Gln (Q)   8	  1.4%
Glu (E)  39	  7.0%
Gly (G)  34	  6.1%
His (H)  14	  2.5%
Ile (I)  30	  5.4%
Leu (L)  59	 10.6%
Lys (K)  36	  6.5%
Met (M)  13	  2.3%
Phe (F)  19	  3.4%
Pro (P)  39	  7.0%
Ser (S)  46	  8.3%
Thr (T)  31	  5.6%
Trp (W)   9	  1.6%
Tyr (Y)  19	  3.4%
Val (V)  40	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2795
Hydrogen    H	      4395
Nitrogen    N	       745
Oxygen      O	       821
Sulfur      S	        20

Formula: C2795H4395N745O821S20
Total number of atoms: 8776

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78185
Abs 0.1% (=1 g/l)   1.257, assuming all pairs of Cys residues form cystines


Ext. coefficient    77810
Abs 0.1% (=1 g/l)   1.251, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.30
This classifies the protein as unstable.



Aliphatic index: 88.85

Grand average of hydropathicity (GRAVY): -0.280