ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAESTTLETI EIHPITFPPE VLARISPELS LQRHLSLGIR PCLRKYEEFR DVAIENNTLS 

        70         80         90        100        110        120 
RYADAGNIDT KNNILGSNVL KSGKTIVITS ITGGIIEETS AAIKDLDDFG EEELFEVTKE 

       130        140        150        160        170        180 
EDIIANYASV YPVVEVERGR VGACTDEEMT ISQKLHDSIL HSRILPKKAL KVKAGVRSAN 

       190        200        210        220        230        240 
EDGTFSVLYP DELEDDTLNE TNLKMKRKWS YVLYAKIVVL SRTGPVFDLC WNSLMYALQS 

       250        260        270        280        290        300 
VKLPRAFIDE RASDLRMTIR TRGRSATIRE TYEIICDQTK SVPLMINAKN IAFASNYGIV 

       310        320        330        340        350        360 
ELDPECQLQN SDNSEEEEVD IDMDKLNTVL IADLDTEAEE TSIHSTISIL AAPSGNYKQL 

       370        380        390 
TLVGGGAKIT PEMIKRSLLL SRVRADDLST RFNI 

References and documentation are available.

Number of amino acids: 394

Molecular weight: 44011.06

Theoretical pI: 4.88

Amino acid composition: 
Ala (A)  27	  6.9%
Arg (R)  23	  5.8%
Asn (N)  19	  4.8%
Asp (D)  26	  6.6%
Cys (C)   5	  1.3%
Gln (Q)   7	  1.8%
Glu (E)  37	  9.4%
Gly (G)  18	  4.6%
His (H)   5	  1.3%
Ile (I)  36	  9.1%
Leu (L)  40	 10.2%
Lys (K)  22	  5.6%
Met (M)   8	  2.0%
Phe (F)   9	  2.3%
Pro (P)  14	  3.6%
Ser (S)  32	  8.1%
Thr (T)  29	  7.4%
Trp (W)   2	  0.5%
Tyr (Y)  11	  2.8%
Val (V)  24	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1932
Hydrogen    H	      3135
Nitrogen    N	       523
Oxygen      O	       619
Sulfur      S	        13

Formula: C1932H3135N523O619S13
Total number of atoms: 6222

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27640
Abs 0.1% (=1 g/l)   0.628, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.622, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.36
This classifies the protein as unstable.



Aliphatic index: 99.75

Grand average of hydropathicity (GRAVY): -0.234