ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAETDVGSVK GKEKGSGKRW ILLIGAIAAV LLAVVVAVFL NTQNSSISEF TGKICNCRQA 

        70         80         90        100        110        120 
EQQKYIGIVE DCCCDYETVN RLNTEVLNPL LQDLVKTPFY RYFKVKLWCD CPFWPDDGMC 

       130        140        150        160        170        180 
RLRDCSVCEC PESEFPEVFK KPLSQYNPVC QEGKPQATVD RTLDTRAFRG WTVTDNPWTS 

       190        200        210        220        230        240 
DDETDNDEMT YVNLRLNPER YTGYIGPSAR RIWEAIYSEN CPKHTSEGSC QEEKILYKLV 

       250        260        270        280        290        300 
SGLHSSISVH IASDYLLDEA TNLWGQNLTL LYDRVLRYPD RVQNLYFTFL FVLRAVTKAE 

       310        320        330        340        350        360 
DYLGEAEYET GNVIEDLKTK SLVKQVVSDP KTKAACPVPF DEAKLWKGQR GPELKQQLEK 

       370        380        390        400        410        420 
QFRNISAIMD CVGCEKCRLW GKLQILGLGT ALKILFTVNG EDNLRHNLEL QRNEVIALMN 

       430        440        450        460        470 
LLHRLSESVK YVHDMSPAAE RIAGGHASSG NSFWQRIVTS IAQSKAVSGK RS 

References and documentation are available.

Number of amino acids: 472

Molecular weight: 53449.01

Theoretical pI: 6.08

Amino acid composition: 
Ala (A)  28	  5.9%
Arg (R)  26	  5.5%
Asn (N)  23	  4.9%
Asp (D)  26	  5.5%
Cys (C)  18	  3.8%
Gln (Q)  20	  4.2%
Glu (E)  35	  7.4%
Gly (G)  28	  5.9%
His (H)   7	  1.5%
Ile (I)  22	  4.7%
Leu (L)  48	 10.2%
Lys (K)  31	  6.6%
Met (M)   6	  1.3%
Phe (F)  15	  3.2%
Pro (P)  19	  4.0%
Ser (S)  31	  6.6%
Thr (T)  26	  5.5%
Trp (W)  10	  2.1%
Tyr (Y)  17	  3.6%
Val (V)  36	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      2369
Hydrogen    H	      3728
Nitrogen    N	       648
Oxygen      O	       712
Sulfur      S	        24

Formula: C2369H3728N648O712S24
Total number of atoms: 7481

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81455
Abs 0.1% (=1 g/l)   1.524, assuming all pairs of Cys residues form cystines


Ext. coefficient    80330
Abs 0.1% (=1 g/l)   1.503, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.36
This classifies the protein as stable.



Aliphatic index: 85.89

Grand average of hydropathicity (GRAVY): -0.336