ProtParam
User-provided sequence:
10 20 30 40 50 60
MAETKQPLIG LKWEPKLPGL SLDLKTGSTR SKTAESSKSE LVDGLCLPPN DPRKINKMIR
70 80 90 100 110 120
KQLKDTTGSN WFDMPAPTMT PELKRDLQLL KLRTVMDPAL HYKKSVSRSK LAEKYFQIGT
130 140 150 160 170 180
VIEPAEEFYG RLTKKNRKAT LADELVSDPK TALYRKRKVR EIEEKSRAVT NKKWNKKGNQ
SKNTKPRRN
References and
documentation are available.
Number of amino acids: 189
Molecular weight: 21680.24
Theoretical pI: 10.13
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 957
Hydrogen H 1593
Nitrogen N 279
Oxygen O 280
Sulfur S 6
Formula: C
957H
1593N
279O
280S
6
Total number of atoms: 3115
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22460
Abs 0.1% (=1 g/l) 1.036, assuming all pairs of Cys residues form cystines
Ext. coefficient 22460
Abs 0.1% (=1 g/l) 1.036, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 55.35
This classifies the protein as unstable.
Aliphatic index: 71.75
Grand average of hydropathicity (GRAVY): -0.971