ProtParam
User-provided sequence:
10 20 30 40 50 60
MAETQGKVIT CKAAVVWGPK VPLVIQEICV DPPQKMEVRV KILYSSICHT DLGCWNGTNE
70 80 90 100 110 120
AERAFPRILG HEAVGIVESV GEGVKDVKEG DYVIPTFNGE CGECKVCKRE ESNLCERYHV
130 140 150 160 170 180
DPMKRVMVND GGTRFSTTIN KDGGSSQSQP IYHFLNTSTF TEYTVLDSAC VVKIDPNSPL
190 200 210 220 230 240
KQMSLLSCGV STGVGAAWNI ANVKEGKSTA VFGLGSVGLA VAEGARARGA SRIIGVDANA
250 260 270 280 290 300
SKFEKGKLMG VTDFINPKDL TKPVHQMIRE ITGGGVDYSF ECTGNVDVLR EAFLSTHVGW
310 320 330 340 350 360
GSTVLVGIYP TPRTLPLHPM ELFDGRRITG SVFGGFKPKS QLPNFAQQCM KGVVKLEPFI
370 380 390
TNELPFEKIN DAFQLLRDGK SLRCILQISK LLKR
References and
documentation are available.
Number of amino acids: 394
Molecular weight: 42907.50
Theoretical pI: 8.34
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1900
Hydrogen H 3038
Nitrogen N 522
Oxygen O 563
Sulfur S 22
Formula: C
1900H
3038N
522O
563S
22
Total number of atoms: 6045
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33180
Abs 0.1% (=1 g/l) 0.773, assuming all pairs of Cys residues form cystines
Ext. coefficient 32430
Abs 0.1% (=1 g/l) 0.756, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.44
This classifies the protein as stable.
Aliphatic index: 85.51
Grand average of hydropathicity (GRAVY): -0.108