ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAEVDISHSK KKKQDKTEND AADTGDYMIK PQSFTPAIDT SQWPILLKNY DRLNVRTGHY 

        70         80         90        100        110        120 
TPISAGHSPL KRPLQEYIRY GVINLDKPAN PSSHEVVAWI KRILRVEKTG HSGTLDPKVT 

       130        140        150        160        170        180 
GNLIVCIDRA TRLVKSQQGA GKEYVCVARL HSAVPDVAKV ARALESLTGA VFQRPPLISA 

       190        200        210        220        230        240 
VKRQLRIRTI YESKLLEYDA DRHLVVFWVS CEAGTYIRTM CVHLGLLLGV GGHMQELRRV 

       250        260        270        280        290        300 
RSGILGENNN MVTMHDVMDA QFVYDNSRDE SYLRRVIMPL EMILTSYKRL VVKDSAVNAI 

       310        320        330        340        350        360 
CYGAKLMIPG LLRFENDIDV GTEVVLMTTK GEAIAVGIAE MTTSVMATCD HGVVAKIKRV 

       370        380        390        400        410        420 
VMDRDTYPRK WGLGPRASMK KKLIADGKLD KHGKPNEKTP VEWSRNVVLP TGGDAIIAGA 

       430        440        450        460        470        480 
AAAPEEIKAD AENGEAGEAR KRKHDDSSDS PAPVTTKKSK TKEVEGEEAE EKVKSSKKKK 

       490        500        510        520        530        540 
KKDKEEEKEE EAGSEKKEKK KKKDKKEEVI EEVASPKSEK KKKKKSKDTE AAVDAEDESA 

       550        560 
AEKSEKKKKK KDKKKKNKDS EDDEE 

References and documentation are available.

Number of amino acids: 565

Molecular weight: 63026.32

Theoretical pI: 9.19

Amino acid composition: 
Ala (A)  48	  8.5%
Arg (R)  31	  5.5%
Asn (N)  16	  2.8%
Asp (D)  39	  6.9%
Cys (C)   6	  1.1%
Gln (Q)  10	  1.8%
Glu (E)  52	  9.2%
Gly (G)  34	  6.0%
His (H)  13	  2.3%
Ile (I)  29	  5.1%
Leu (L)  36	  6.4%
Lys (K)  77	 13.6%
Met (M)  15	  2.7%
Phe (F)   5	  0.9%
Pro (P)  23	  4.1%
Ser (S)  36	  6.4%
Thr (T)  29	  5.1%
Trp (W)   5	  0.9%
Tyr (Y)  14	  2.5%
Val (V)  47	  8.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 108

Atomic composition:

Carbon      C	      2751
Hydrogen    H	      4534
Nitrogen    N	       792
Oxygen      O	       853
Sulfur      S	        21

Formula: C2751H4534N792O853S21
Total number of atoms: 8951

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48735
Abs 0.1% (=1 g/l)   0.773, assuming all pairs of Cys residues form cystines


Ext. coefficient    48360
Abs 0.1% (=1 g/l)   0.767, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.58
This classifies the protein as stable.



Aliphatic index: 77.49

Grand average of hydropathicity (GRAVY): -0.716