ProtParam
User-provided sequence:
10 20 30 40 50 60
MAEVEEIAHE EQNIEKREKK KGKHEKPKPW DDDPNIDRWT IEKFDPAWNP TGMTETSTFS
70 80 90 100 110 120
TLFPQYREKY LQECWPRVES ALKEYGVACK LNLVEGSMTV STTRKTRDPY IIVKARDLIK
130 140 150 160 170 180
LLSRSVPAPQ AIKILEDEVQ CDIIKIGNLV RNKERFVKRR QRLVGPNSST LKALEILTNC
190 200 210 220 230 240
YILVQGSTVA AMGPFKGLKQ LRRIVEDCVQ NIMHPVYHIK TLMMKKELEK DPALANESWD
250 260 270 280 290 300
RFLPTFRKKN VKQKKPKSKE KKPYTPFPPP QPPSKIDMQL ESGEYFMSDK KKSEKKWQEK
310 320 330 340 350 360
QEKQSEKSTE NKRKRDASFL PPEEPMNNNS NANKSEDGKN DITELTNSLK SKTKELKKQK
370 380 390
KTHERVNAEE YIAGPSSSAD KSSKKSKKIR D
References and
documentation are available.
Number of amino acids: 391
Molecular weight: 45323.08
Theoretical pI: 9.53
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 80
Atomic composition:
Carbon C 1999
Hydrogen H 3245
Nitrogen N 565
Oxygen O 603
Sulfur S 15
Formula: C
1999H
3245N
565O
603S
15
Total number of atoms: 6427
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46660
Abs 0.1% (=1 g/l) 1.029, assuming all pairs of Cys residues form cystines
Ext. coefficient 46410
Abs 0.1% (=1 g/l) 1.024, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.50
This classifies the protein as unstable.
Aliphatic index: 64.83
Grand average of hydropathicity (GRAVY): -1.053