ProtParam
User-provided sequence:
10 20 30 40 50 60
MAEVPHHNIP AVDMLNATSR LQLEAQELRN NKPNWGSYFR SQMIQEDDYN FITSFENAKS
70 80 90 100 110 120
KEERDQVLAA NNANGQAAKT MANLITQVAK DQNVRYVLTL FDDMLQEDKS RVELFHSAAA
130 140 150 160 170 180
RQKRTVWSQY LGILQRQDNF IVNQMSSIIA KLACFGTTRM EGQDLQYYFS FLKEQLKNST
190 200 210 220 230 240
TNDYMNTTAR CLQMMLRHDE YRHEFVDSDG VQTLVTALNG KTNFQLQYQL IFAVWCLTFN
250 260 270 280 290 300
ADIARKAPSL GLIQALGDIL SESTKEKVIR IILASFVNIL SKVDEREVKR EAALQMVQCK
310 320 330 340 350 360
TLKTLELMDA KKYDDPDLED DVKFLTEELT LSVHDLSSYD EYYSEVRSGR LQWSPVHKSE
370 380 390 400 410 420
KFWRENASKF NDKQFEVVKI LIKLLESSHD PLILCVASHD IGEYVRHYPR GKTVVEQYQG
430 440 450 460 470
KAAVMRLLTA EDPNVRYHAL LAVQKLMVHN WEYLGKQLDS DVQTDTVAVK
References and
documentation are available.
Number of amino acids: 470
Molecular weight: 54212.65
Theoretical pI: 5.90
Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2403
Hydrogen H 3789
Nitrogen N 661
Oxygen O 729
Sulfur S 19
Formula: C
2403H
3789N
661O
729S
19
Total number of atoms: 7601
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 60070
Abs 0.1% (=1 g/l) 1.108, assuming all pairs of Cys residues form cystines
Ext. coefficient 59820
Abs 0.1% (=1 g/l) 1.103, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.01
This classifies the protein as unstable.
Aliphatic index: 88.17
Grand average of hydropathicity (GRAVY): -0.425