ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAEYLASIFG TEKDKVNCSF YFKIGACRHG DRCSRIHNKP TFSQTVLLQN LYVNPQNSAK 

        70         80         90        100        110        120 
SADGSHLVAN VSDEEMQEHY DNFFEDVFVE CEDKYGEIEE MNVCDNLGDH LVGNVYIKFR 

       130        140        150        160        170        180 
NEADAEKAAN DLNNRWFGGR PVYSELSPVT DFREACCRQY EMGECTRSGF CNFMHLKPIS 

       190        200        210        220        230        240 
RELRRYLYSR RRRARSRSRS PGRRRGSRSR SRSPGRRGGG RGDGVGGGNY LNNERDNMRG 

       250        260 
NDRGNDRDRR KGGGGGGGGG GGRY 

References and documentation are available.

Number of amino acids: 264

Molecular weight: 29876.97

Theoretical pI: 9.15

Amino acid composition: 
Ala (A)  12	  4.5%
Arg (R)  35	 13.3%
Asn (N)  21	  8.0%
Asp (D)  17	  6.4%
Cys (C)   9	  3.4%
Gln (Q)   5	  1.9%
Glu (E)  19	  7.2%
Gly (G)  35	 13.3%
His (H)   6	  2.3%
Ile (I)   6	  2.3%
Leu (L)  13	  4.9%
Lys (K)  10	  3.8%
Met (M)   6	  2.3%
Phe (F)  12	  4.5%
Pro (P)   7	  2.7%
Ser (S)  20	  7.6%
Thr (T)   5	  1.9%
Trp (W)   1	  0.4%
Tyr (Y)  12	  4.5%
Val (V)  13	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1262
Hydrogen    H	      1968
Nitrogen    N	       418
Oxygen      O	       400
Sulfur      S	        15

Formula: C1262H1968N418O400S15
Total number of atoms: 4063

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23880
Abs 0.1% (=1 g/l)   0.799, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.783, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 77.64
This classifies the protein as unstable.



Aliphatic index: 46.89

Grand average of hydropathicity (GRAVY): -1.037