ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFEALTGIN GDLITRSWSA SKQAYLTERY HKEEAGAVVI FAFQPSFSEK DFFDPDNKSS 

        70         80         90        100        110        120 
FGEIKLNRVQ FPCMRKIGKG DVATVNEAFL KNLEAIIDPR TSFQASVEMA VRSRKQIVFT 

       130        140        150        160        170        180 
GHSSGGATAI LATVWYLEKY FIRNPNVYLE PRCVTFGAPL VGDSIFSHAL GREKWSRFFV 

       190        200        210        220        230        240 
NFVSRFDIVP RIMLARKASV EETLPHVLAQ LDPRKSSVQE SEQRITEFYT RVMRDTSTVA 

       250        260        270        280        290        300 
NQAVCELTGS AEAFLETLSS FLELSPYRPA GTFVFSTEKR LVAVNNSDAI LQMLFYTSQA 

       310        320        330        340        350        360 
SDEQEWSLIP FRSIRDHHSY EELVQSMGKK LFNHLDGENS IESTLNDLGV STRGRQYVQA 

       370        380        390        400        410        420 
ALEEEKKRVE NQKKIIQVIE QERFLKKLAW IEDEYKPKCQ AHKNGYYDSF KVSNEENDFK 

       430        440        450        460        470        480 
ANVKRAELAG VFDEVLGLMK KCQLPDEFEG DIDWIKLATR YRRLVEPLDI ANYHRHLKNE 

       490        500        510        520        530        540 
DTGPYMKRGR PTRYIYAQRG YEHYILKPNG MIAEDVFWNK VNGLNLGLQL EEIQETLKNS 

       550        560        570        580        590        600 
GSECGSCFWA EVEELKGKPY EEVEVRVKTL EGMLGEWITD GEVDDKEIFL EGSTFRKWWI 

       610        620 
TLPKNHKSHS PLRDYMMDEI TDT 

References and documentation are available.

Number of amino acids: 623

Molecular weight: 71690.27

Theoretical pI: 5.73

Amino acid composition: 
Ala (A)  40	  6.4%
Arg (R)  38	  6.1%
Asn (N)  27	  4.3%
Asp (D)  31	  5.0%
Cys (C)   7	  1.1%
Gln (Q)  23	  3.7%
Glu (E)  62	 10.0%
Gly (G)  35	  5.6%
His (H)  13	  2.1%
Ile (I)  33	  5.3%
Leu (L)  52	  8.3%
Lys (K)  43	  6.9%
Met (M)  13	  2.1%
Phe (F)  35	  5.6%
Pro (P)  22	  3.5%
Ser (S)  44	  7.1%
Thr (T)  31	  5.0%
Trp (W)  11	  1.8%
Tyr (Y)  22	  3.5%
Val (V)  41	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 81

Atomic composition:

Carbon      C	      3212
Hydrogen    H	      4975
Nitrogen    N	       867
Oxygen      O	       957
Sulfur      S	        20

Formula: C3212H4975N867O957S20
Total number of atoms: 10031

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    93655
Abs 0.1% (=1 g/l)   1.306, assuming all pairs of Cys residues form cystines


Ext. coefficient    93280
Abs 0.1% (=1 g/l)   1.301, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.16
This classifies the protein as stable.



Aliphatic index: 78.72

Grand average of hydropathicity (GRAVY): -0.473