ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFEFKLPDI GEGIHEGEIV KWFIKPGDEV NEDDVLLEVQ NDKAVVEIPS PVKGKVLEVL 

        70         80         90        100        110        120 
VEEGTVAVVG DTLIKFDAPG YENLKFKGDD HDEAPKAEAT PAATAEVVNE RVIAMPSVRK 

       130        140        150        160        170        180 
YARENGVDIH KVAGSGKNGR IVKADIDAFA NGGQAVAATE APAAVEATPA AAKEEAPKAQ 

       190        200        210        220        230        240 
PIPAGEYPET REKMSGIRKA IAKAMVNSKH TAPHVTLMDE VDVTELVAHR KKFKAVAADK 

       250        260        270        280        290        300 
GIKLTYLPYV VKALTSALRE YPMLNTSLDD ASQEVVHKHY FNIGIAADTD KGLLVPVVKD 

       310        320        330        340        350        360 
TDRKSIFTIS NEINDLAGKA REGRLAPAEM KGASCTITNI GSAGGQWFTP VINHPEVAIL 

       370        380        390        400        410 
GIGRIAEKPV VKNGEIVAAP VLALSLSFDH RLIDGATAQK ALNQIKRLLN DPQLLVMEA 

References and documentation are available.

Number of amino acids: 419

Molecular weight: 44901.46

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  57	 13.6%
Arg (R)  14	  3.3%
Asn (N)  17	  4.1%
Asp (D)  26	  6.2%
Cys (C)   1	  0.2%
Gln (Q)   8	  1.9%
Glu (E)  35	  8.4%
Gly (G)  30	  7.2%
His (H)  10	  2.4%
Ile (I)  28	  6.7%
Leu (L)  29	  6.9%
Lys (K)  36	  8.6%
Met (M)   8	  1.9%
Phe (F)  11	  2.6%
Pro (P)  24	  5.7%
Ser (S)  14	  3.3%
Thr (T)  20	  4.8%
Trp (W)   2	  0.5%
Tyr (Y)   7	  1.7%
Val (V)  42	 10.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      1999
Hydrogen    H	      3230
Nitrogen    N	       544
Oxygen      O	       608
Sulfur      S	         9

Formula: C1999H3230N544O608S9
Total number of atoms: 6390

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.477, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.477, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.65
This classifies the protein as stable.



Aliphatic index: 95.73

Grand average of hydropathicity (GRAVY): -0.141