ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFEKIKVAN PIVEMDGDEM TRVIWKSIKD KLITPFVELD IKYFDLGLPH RDATDDKVTI 

        70         80         90        100        110        120 
ESAEATKKYN VAIKCATITP DEGRVTEFGL KQMWRSPNGT IRNILNGTVF REPIICKNVP 

       130        140        150        160        170        180 
KLVPGWTKPI CIGRHAFGDQ YRATDAVIKG PGKLTMTFEG KDGKTETEVF TFTGEGGVAM 

       190        200        210        220        230        240 
AMYNTDESIR AFADASMNTA YEKKWPLYLS TKNTILKKYD GRFKDIFQEV YEASWKSKYD 

       250        260        270        280        290        300 
AAGIWYEHRL IDDMVAYALK SEGGYVWACK NYDGDVQSDF LAQGFGSLGL MTSVLVCPDG 

       310        320        330        340        350        360 
KTIEAEAAHG TVTRHFRVHQ KGGETSTNSI ASIFAWTRGL AHRAKLDDNA KLLDFTEKLE 

       370        380        390        400        410 
AACVGTVESG KMTKDLALII HGSKLSRDTY LNTEEFIDAV AAELKERLNA 

References and documentation are available.

Number of amino acids: 410

Molecular weight: 45746.22

Theoretical pI: 6.13

Amino acid composition: 
Ala (A)  38	  9.3%
Arg (R)  17	  4.1%
Asn (N)  14	  3.4%
Asp (D)  29	  7.1%
Cys (C)   6	  1.5%
Gln (Q)   6	  1.5%
Glu (E)  29	  7.1%
Gly (G)  32	  7.8%
His (H)   8	  2.0%
Ile (I)  27	  6.6%
Leu (L)  28	  6.8%
Lys (K)  37	  9.0%
Met (M)  11	  2.7%
Phe (F)  18	  4.4%
Pro (P)  12	  2.9%
Ser (S)  18	  4.4%
Thr (T)  34	  8.3%
Trp (W)   8	  2.0%
Tyr (Y)  14	  3.4%
Val (V)  24	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2046
Hydrogen    H	      3202
Nitrogen    N	       542
Oxygen      O	       613
Sulfur      S	        17

Formula: C2046H3202N542O613S17
Total number of atoms: 6420

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65235
Abs 0.1% (=1 g/l)   1.426, assuming all pairs of Cys residues form cystines


Ext. coefficient    64860
Abs 0.1% (=1 g/l)   1.418, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 19.81
This classifies the protein as stable.



Aliphatic index: 78.56

Grand average of hydropathicity (GRAVY): -0.321