ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFGRNNRVR FRDWISEGTE YGYGRNKARP SLNTVLKNVR RGLKKPLSFG SHNKKRDSNS 

        70         80         90        100        110        120 
STTTQKNIIN PQGSFLQNWN KIFLFASVIA LAIDPLFFYI PIVDGERHCL NLHRNLEIAA 

       130        140        150        160        170        180 
SVLRTFIDAF YIIHIVFQFR TAYISPSSRV FGRGELVDDP KAIAIKYLSS YFIIDLLSIL 

       190        200        210        220        230        240 
PLPQLVVLAV IPNVNKPVSL ITKDYLITVI FTQYIPRILR IYPLYTEVTR TSGIVTETAW 

       250        260        270        280        290        300 
AGAAWNLSLY MLASHVFGAL WYLISVERED RCWREACEKI PEVCNFRFLY CDGNSSVRND 

       310        320        330        340        350        360 
FLTTSCPFIN PDDITNSTVF NFGIFTDALK SGIVESDDFW KKFFYCFWWG LRNLSALGQN 

       370        380        390        400        410        420 
LNTSKFVGEI IFAVSICISG LVLFALLIGN MQKYLESTTV REEEMRVRKR DAEQWMSHRM 

       430        440        450        460        470        480 
LPDDLRKRIR RYEQYKWQET RGVEEENLLR NLPKDLRRDI KRHFCLDLLK KVPLFEIMDE 

       490        500        510        520        530        540 
QLLDAVCDKL KPVLYTENSY AIREGDPVEE MLFVMRGKLM SATTNGGRTG FFNAVYLKPS 

       550        560        570        580        590        600 
DFCGEDLLTW ALDPQSSSHF PISTRTVQAL TEVEAFALAA DDLKLVASQF RRLHSKQLQH 

       610        620        630        640        650        660 
TFRFYSVQWR TWGASFIQAA WRRHCRRKLA RSLTEEEDRF RNAITKRERN AASSSSLVAT 

       670        680        690 
LYASRFASNA LRNLRTNNLP LLPPKPSEPD FSLRNP 

References and documentation are available.

Number of amino acids: 696

Molecular weight: 80473.68

Theoretical pI: 9.39

Amino acid composition: 
Ala (A)  46	  6.6%
Arg (R)  59	  8.5%
Asn (N)  38	  5.5%
Asp (D)  34	  4.9%
Cys (C)  12	  1.7%
Gln (Q)  19	  2.7%
Glu (E)  38	  5.5%
Gly (G)  29	  4.2%
His (H)  11	  1.6%
Ile (I)  45	  6.5%
Leu (L)  77	 11.1%
Lys (K)  34	  4.9%
Met (M)  10	  1.4%
Phe (F)  45	  6.5%
Pro (P)  30	  4.3%
Ser (S)  53	  7.6%
Thr (T)  38	  5.5%
Trp (W)  15	  2.2%
Tyr (Y)  23	  3.3%
Val (V)  40	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 93

Atomic composition:

Carbon      C	      3649
Hydrogen    H	      5683
Nitrogen    N	      1001
Oxygen      O	      1012
Sulfur      S	        22

Formula: C3649H5683N1001O1012S22
Total number of atoms: 11367

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   117520
Abs 0.1% (=1 g/l)   1.460, assuming all pairs of Cys residues form cystines


Ext. coefficient   116770
Abs 0.1% (=1 g/l)   1.451, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.34
This classifies the protein as unstable.



Aliphatic index: 91.64

Grand average of hydropathicity (GRAVY): -0.195