ProtParam
User-provided sequence:
10 20 30 40 50 60
MAFNFTAFTY IVALIGDAFL IFFAIFHVIA FDELKTDYKN PIDQCNSLNP LVLPEYLLHI
70 80 90 100 110 120
FLNLLFLFCG EWFSLCINIP LIAYHIWRYK NRPVMSGPGL YDPTTVLKTD TLYRNMREGW
130 140
IKLAVYLISF FYYIYGMVYS LIST
References and
documentation are available.
Number of amino acids: 144
Molecular weight: 16931.00
Theoretical pI: 6.25
Total number of negatively charged residues (Asp + Glu): 10
Total number of positively charged residues (Arg + Lys): 9
Atomic composition:
Carbon C 815
Hydrogen H 1201
Nitrogen N 179
Oxygen O 200
Sulfur S 7
Formula: C
815H
1201N
179O
200S
7
Total number of atoms: 2402
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34505
Abs 0.1% (=1 g/l) 2.038, assuming all pairs of Cys residues form cystines
Ext. coefficient 34380
Abs 0.1% (=1 g/l) 2.031, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 24.82
This classifies the protein as stable.
Aliphatic index: 114.44
Grand average of hydropathicity (GRAVY): 0.595