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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFNFTAFTY IVALIGDAFL IFFAIFHVIA FDELKTDYKN PIDQCNSLNP LVLPEYLLHI

70 80 90 100 110 120
FLNLLFLFCG EWFSLCINIP LIAYHIWRYK NRPVMSGPGL YDPTTVLKTD TLYRNMREGW

130 140
IKLAVYLISF FYYIYGMVYS LIST


References and documentation are available.

Number of amino acids: 144 Molecular weight: 16931.00 Theoretical pI: 6.25
Amino acid composition: 
Ala (A) 8 5.6% Arg (R) 4 2.8% Asn (N) 8 5.6% Asp (D) 6 4.2% Cys (C) 3 2.1% Gln (Q) 1 0.7% Glu (E) 4 2.8% Gly (G) 6 4.2% His (H) 3 2.1% Ile (I) 15 10.4% Leu (L) 20 13.9% Lys (K) 5 3.5% Met (M) 4 2.8% Phe (F) 14 9.7% Pro (P) 7 4.9% Ser (S) 6 4.2% Thr (T) 8 5.6% Trp (W) 3 2.1% Tyr (Y) 12 8.3% Val (V) 7 4.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 10 Total number of positively charged residues (Arg + Lys): 9 Atomic composition: Carbon C 815 Hydrogen H 1201 Nitrogen N 179 Oxygen O 200 Sulfur S 7 Formula: C815H1201N179O200S7 Total number of atoms: 2402 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 34505 Abs 0.1% (=1 g/l) 2.038, assuming all pairs of Cys residues form cystines Ext. coefficient 34380 Abs 0.1% (=1 g/l) 2.031, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 24.82 This classifies the protein as stable. Aliphatic index: 114.44 Grand average of hydropathicity (GRAVY): 0.595