ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFQKDAKSS AYSSRFQTPF RRRREGKTDY YQRKRLVTQH KAKYNTPKYR LVVRFTNKDI 

        70         80         90        100        110        120 
ICQIISSTIT GDVVLAAAYS HELPRYGITH GLTNWAAAYA TGLLIARRTL QKLGLDETYK 

       130        140        150        160        170        180 
GVEEVEGEYE LTEAVEDGPR PFKVFLDIGL QRTTTGARVF GALKGASDGG LYVPHSENRF 

       190        200        210        220        230        240 
PGWDFETEEI DPELLRSYIF GGHVSQYMEE LADDDEERFS ELFKGYLADD IDADSLEDIY 

       250        260        270        280        290 
TSAHEAIRAD PAFKPTEKKF TKEQYAAESK KYRQTKLSKE ERAARVAAKI AALAGQQ 

References and documentation are available.

Number of amino acids: 297

Molecular weight: 33714.82

Theoretical pI: 6.36

Amino acid composition: 
Ala (A)  32	 10.8%
Arg (R)  22	  7.4%
Asn (N)   4	  1.3%
Asp (D)  19	  6.4%
Cys (C)   1	  0.3%
Gln (Q)  12	  4.0%
Glu (E)  27	  9.1%
Gly (G)  20	  6.7%
His (H)   6	  2.0%
Ile (I)  14	  4.7%
Leu (L)  23	  7.7%
Lys (K)  22	  7.4%
Met (M)   2	  0.7%
Phe (F)  14	  4.7%
Pro (P)  10	  3.4%
Ser (S)  16	  5.4%
Thr (T)  21	  7.1%
Trp (W)   2	  0.7%
Tyr (Y)  17	  5.7%
Val (V)  13	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1506
Hydrogen    H	      2339
Nitrogen    N	       415
Oxygen      O	       460
Sulfur      S	         3

Formula: C1506H2339N415O460S3
Total number of atoms: 4723

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36330
Abs 0.1% (=1 g/l)   1.078, assuming all pairs of Cys residues form cystines


Ext. coefficient    36330
Abs 0.1% (=1 g/l)   1.078, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.04
This classifies the protein as stable.



Aliphatic index: 72.05

Grand average of hydropathicity (GRAVY): -0.634