ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFQQGVLSR CSGVFRHHVG HSRHINNILY RHAIAFASIA PRIPKSSFHT SAIRNNEAFK 

        70         80         90        100        110        120 
DPYDTLGLKK SATGAEIKKA YYKLAKKYHP DINKEPDAEK KFHDLQNAYE ILSDETKRQQ 

       130        140        150        160        170        180 
YDQFGPAAFG GGGAAGGAGG GSGSPFGSQF HDFSGFTSAG GSPFGGINFE DLFGAAFGGG 

       190        200        210        220        230        240 
GRGSGGASRS SSMFRQYRGD PIEIVHKVSF KDAVFGSKNV QLRFSALDPC STCSGTGMKP 

       250        260        270        280        290        300 
NTHKVSCSTC HGTGTTVHIR GGFQMMSTCP TCNGEGTMKR PQDNCTKCHG EGVQVNRAKT 

       310        320        330        340        350        360 
ITVDLPHGLQ DGDVVRIPGQ GSYPDIAVEA DLKDSVKLSR GDILVRIRVD KDPNFSIKNK 

       370        380        390        400        410        420 
YDIWYDKEIP ITTAALGGTV TIPTVEGQKI RIKVAPGTQY NQVISIPNMG VPKTSTIRGD 

       430        440        450        460        470        480 
MKVQYKIVVK KPQSLAEKCL WEALADVTND DMAKKTMQPG TAAGTAINEE ILKKQKQEEE 

       490        500        510 
KHAKKDDDNT LKRLENFITN TFRKIKGDKK N 

References and documentation are available.

Number of amino acids: 511

Molecular weight: 55560.88

Theoretical pI: 9.31

Amino acid composition: 
Ala (A)  38	  7.4%
Arg (R)  23	  4.5%
Asn (N)  22	  4.3%
Asp (D)  32	  6.3%
Cys (C)  10	  2.0%
Gln (Q)  22	  4.3%
Glu (E)  21	  4.1%
Gly (G)  56	 11.0%
His (H)  16	  3.1%
Ile (I)  33	  6.5%
Leu (L)  22	  4.3%
Lys (K)  48	  9.4%
Met (M)  10	  2.0%
Phe (F)  24	  4.7%
Pro (P)  24	  4.7%
Ser (S)  35	  6.8%
Thr (T)  33	  6.5%
Trp (W)   2	  0.4%
Tyr (Y)  13	  2.5%
Val (V)  27	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2436
Hydrogen    H	      3854
Nitrogen    N	       706
Oxygen      O	       743
Sulfur      S	        20

Formula: C2436H3854N706O743S20
Total number of atoms: 7759

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30995
Abs 0.1% (=1 g/l)   0.558, assuming all pairs of Cys residues form cystines


Ext. coefficient    30370
Abs 0.1% (=1 g/l)   0.547, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.86
This classifies the protein as stable.



Aliphatic index: 64.74

Grand average of hydropathicity (GRAVY): -0.561