ProtParam
User-provided sequence:
10 20 30 40 50 60
MAFSMLFTGH AECGGYTKEN VARCWTVETG ILLDTELPNE IPENDYIKYF APDFSLKIPG
70 80 90 100 110 120
GHIENLNTKS YISSIKVQIL ENLRYIQHAP SVQMQEVPPD FYIPDFDEDE QNPDVRVDQR
130 140
SRDKQIQRDD EYFDGDNDND AS
References and
documentation are available.
Number of amino acids: 142
Molecular weight: 16384.03
Theoretical pI: 4.29
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 12
Atomic composition:
Carbon C 722
Hydrogen H 1090
Nitrogen N 190
Oxygen O 237
Sulfur S 5
Formula: C
722H
1090N
190O
237S
5
Total number of atoms: 2244
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16055
Abs 0.1% (=1 g/l) 0.980, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.972, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.27
This classifies the protein as stable.
Aliphatic index: 70.70
Grand average of hydropathicity (GRAVY): -0.739