ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAFSSPSDFK RYHVFSSFHG PDVRSGFLSH LHNHFESKGI TPFKDQEIER GHTIGPELIQ 

        70         80         90        100        110        120 
AIRESRVSIV VLSEKYASSC WCLDELVEIL KCKEASGQVV MTIFYKVDPS DVRKQRGDFG 

       130        140        150        160        170        180 
STFKKTCEGK TWIVKQRWIK ALEYIATVAG EHSLSWANEA ELIQKIATDV SNKLNLTPSR 

       190        200        210        220        230        240 
DFEGMVGLEA HLTKLDSFLC LESDDVKMIG IWGPAGIGKT TIARALFNQL STGFRLSCFM 

       250        260        270        280        290        300 
GTIDVNDYDS KLCLQNKLLS KILNQKDMKI HHLGAIEEWL HNQRVLIVLD DVDDLEQLEV 

       310        320        330        340        350        360 
LAKESSWFGH GSRIIVSLND RKILKAHGIN DIYDVDFPSE EEALEILCLS AFKQNSPQDG 

       370        380        390        400        410        420 
FEEVAKRVVE LCGKLPLGLR VVGSSFYGES EDEWRIQLYG IETNLDRKIE NVLRVGYDKL 

       430        440        450        460        470        480 
SERHQSLFLH IACFFNHKSV DYVTTMLADS TLDVENGLKT LAAKSLVSTN GWITMHCLLQ 

       490        500        510        520        530        540 
QLGRQVVVQQ GDPGKRQFLV EAKEIRDVLA NETGTESVIG ISFDISKIET LSISKRAFNR 

       550        560        570        580        590        600 
MRNLKFLNFY NGSVSLLEDM EYLPRLRLLY WGSYPRKSLP LTFKPECLVE LYMGFSKLEK 

       610        620        630        640        650        660 
LWGGIQPLTN LKKINLGYSS NLKEIPNLSK ATNLKTLTLT GCESLVEIPS SIWNLQKLEM 

       670        680        690        700        710        720 
LYASGCIKLQ VIPTNINLAS LEEVNMSNCS RLRSFPDISS NIKRLYVAGT MIKEFPASIV 

       730        740        750        760        770        780 
GHWCRLDFLQ IGSRSLKRLT HVPESVTHLD LRNSDIKMIP DCVIGLPHLV SLLVENCTKL 

       790        800        810        820        830        840 
VSIQGHSPSL VTLFADHCIS LKSVCCSFHG PISKLMFYNC LKLDKESKRG IIQQSGNKSI 

       850        860        870        880        890        900 
CLPGKEIPAE FTHQTIGNLI TISLAPGCEE AYSTFSRFKA CLLLSPIKNF AFNKINCFLR 

       910        920        930        940        950        960 
SKGVEISRTT ESIYPFVSGG SLSEHLFIFC GDLFPEENRS LMDVTPNEIL FDFSSSDVEI 

       970        980        990       1000       1010       1020 
VECGVKIFLS SGIEVGYCET GGNRNHHIDG EAEAFKVTQV ENSKHTGHWS WLRKLRLGKK 

      1030       1040       1050       1060       1070       1080 
KMKNNKTELS SRPVSGFSDL TRGIFIRYQE PEAVELTKDE RITDLSSLLC IGSLVVCFFL 

      1090       1100 
SVFFGQPLVV SVLLLFSCFF LQMQ 

References and documentation are available.

Number of amino acids: 1104

Molecular weight: 124314.53

Theoretical pI: 6.77

Amino acid composition: 
Ala (A)  41	  3.7%
Arg (R)  48	  4.3%
Asn (N)  48	  4.3%
Asp (D)  50	  4.5%
Cys (C)  32	  2.9%
Gln (Q)  34	  3.1%
Glu (E)  78	  7.1%
Gly (G)  71	  6.4%
His (H)  30	  2.7%
Ile (I)  79	  7.2%
Leu (L) 135	 12.2%
Lys (K)  76	  6.9%
Met (M)  19	  1.7%
Phe (F)  59	  5.3%
Pro (P)  36	  3.3%
Ser (S) 109	  9.9%
Thr (T)  50	  4.5%
Trp (W)  15	  1.4%
Tyr (Y)  23	  2.1%
Val (V)  71	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 128
Total number of positively charged residues (Arg + Lys): 124

Atomic composition:

Carbon      C	      5581
Hydrogen    H	      8833
Nitrogen    N	      1481
Oxygen      O	      1625
Sulfur      S	        51

Formula: C5581H8833N1481O1625S51
Total number of atoms: 17571

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   118770
Abs 0.1% (=1 g/l)   0.955, assuming all pairs of Cys residues form cystines


Ext. coefficient   116770
Abs 0.1% (=1 g/l)   0.939, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.83
This classifies the protein as unstable.



Aliphatic index: 97.96

Grand average of hydropathicity (GRAVY): -0.066