ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGATSSIIR ENDFEDELAE SMQSYNRETA DKLALTRTES VKPEPEITAP PHSRFSRSFK 

        70         80         90        100        110        120 
TVLIAQCAFT GFFSTIAGAI YYPVLSVIER KFDIDEELVN VTVVVYFVFQ GLAPTFMGGF 

       130        140        150        160        170        180 
ADSLGRRPVV LVAIVIYFGA CIGLACAQTY AQIIVLRCLQ AAGISPVIAI NSGIMGDVTT 

       190        200        210        220        230        240 
RAERGGYVGY VAGFQVLGSA FGALIGAGLS SRWGWRAIFW FLAIGSGICF LASFLILPET 

       250        260        270        280        290        300 
KRNISGNGSV TPKSYLNRAP ILVLPTVRKS LHLDNPDYET LELPTQLNLL APFKILKAYE 

       310        320        330        340        350        360 
ICILMLVAGL QFAMYTTHLT ALSTALSKQY HLTVAKVGLC YLPSGICTLC SIVIAGRYLN 

       370        380        390        400        410        420 
WNYRRRLKYY QNWLGKKRSK LLEEHDNDLN LVQRIIENDP KYTFNIFKAR LQPAFVTLLL 

       430        440        450        460        470        480 
SSSGFCAYGW CITVKAPLAA VLCMSGFASL FSNCILTFST TLIVDLFPTK TSTATGCLNL 

       490        500        510        520        530        540 
FRCILSAVFI AALSKMVEKM KFGGVFTFLG ALTSSSSILL FILLRKGKEL AFKRKKQELG 


VN 

References and documentation are available.

Number of amino acids: 542

Molecular weight: 59618.02

Theoretical pI: 9.26

Amino acid composition: 
Ala (A)  50	  9.2%
Arg (R)  26	  4.8%
Asn (N)  19	  3.5%
Asp (D)  13	  2.4%
Cys (C)  15	  2.8%
Gln (Q)  14	  2.6%
Glu (E)  22	  4.1%
Gly (G)  39	  7.2%
His (H)   5	  0.9%
Ile (I)  40	  7.4%
Leu (L)  69	 12.7%
Lys (K)  27	  5.0%
Met (M)   9	  1.7%
Phe (F)  35	  6.5%
Pro (P)  20	  3.7%
Ser (S)  41	  7.6%
Thr (T)  36	  6.6%
Trp (W)   6	  1.1%
Tyr (Y)  20	  3.7%
Val (V)  36	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2736
Hydrogen    H	      4316
Nitrogen    N	       696
Oxygen      O	       743
Sulfur      S	        24

Formula: C2736H4316N696O743S24
Total number of atoms: 8515

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63675
Abs 0.1% (=1 g/l)   1.068, assuming all pairs of Cys residues form cystines


Ext. coefficient    62800
Abs 0.1% (=1 g/l)   1.053, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.79
This classifies the protein as unstable.



Aliphatic index: 106.92

Grand average of hydropathicity (GRAVY): 0.411