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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGDSDNVSL PKATVQKMIS EILDQDLMFT KDAREIIINS GIEFIMILSS MASEMADNEA

70 80 90 100 110 120
KKTIAPEHVI KALEELEYNE FIPFLEEILL NFKGSQKVKE TRDSKFKKSG LSEEELLRQQ

130 140
EELFRQSRSR LHHNSVSDPV KSEDSS


References and documentation are available.

Number of amino acids: 146 Molecular weight: 16649.87 Theoretical pI: 4.89
Amino acid composition: 
Ala (A) 8 5.5% Arg (R) 6 4.1% Asn (N) 6 4.1% Asp (D) 9 6.2% Cys (C) 0 0.0% Gln (Q) 6 4.1% Glu (E) 19 13.0% Gly (G) 4 2.7% His (H) 3 2.1% Ile (I) 12 8.2% Leu (L) 14 9.6% Lys (K) 13 8.9% Met (M) 6 4.1% Phe (F) 7 4.8% Pro (P) 4 2.7% Ser (S) 18 12.3% Thr (T) 4 2.7% Trp (W) 0 0.0% Tyr (Y) 1 0.7% Val (V) 6 4.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 28 Total number of positively charged residues (Arg + Lys): 19 Atomic composition: Carbon C 727 Hydrogen H 1177 Nitrogen N 195 Oxygen O 238 Sulfur S 6 Formula: C727H1177N195O238S6 Total number of atoms: 2343 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 1490 Abs 0.1% (=1 g/l) 0.089 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 68.86 This classifies the protein as unstable. Aliphatic index: 86.85 Grand average of hydropathicity (GRAVY): -0.521