ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGGEAGVTL GQPHLSRQDL ATLDVTKLTP LSHEVISRQA TINIGTIGHV AHGKSTVVKA 

        70         80         90        100        110        120 
ISGVHTVRFK NELERNITIK LGYANAKIYK LDDPSCPRPE CYRSCGSSTP DEFPTDIPGT 

       130        140        150        160        170        180 
KGNFKLVRHV SFVDCPGHDI LMATMLNGAA VMDAALLLIA GNESCPQPQT SEHLAAIEIM 

       190        200        210        220        230        240 
KLKHILILQN KIDLVKESQA KEQYEQILAF VQGTVAEGAP IIPISAQLKY NIEVVCEYIV 

       250        260        270        280        290        300 
KKIPVPPRDF TSEPRLIVIR SFDVNKPGCE VDDLKGGVAG GSILKGVLKV GQEIEVRPGI 

       310        320        330        340        350        360 
VSKDSEGKLM CKPIFSKIVS LFAEHNDLQY AAPGGLIGVG TKIDPTLCRA DRMVGQVLGA 

       370        380        390        400        410        420 
VGALPEIFTE LEISYFLLRR LLGVRTEGDK KAAKVQKLSK NEVLMVNIGS LSTGGRVSAV 

       430        440        450        460        470 
KADLGKIVLT NPVCTEVGEK IALSRRVEKH WRLIGWGQIR RGVTIKPTVD DD 

References and documentation are available.

Number of amino acids: 472

Molecular weight: 51079.47

Theoretical pI: 8.66

Amino acid composition: 
Ala (A)  33	  7.0%
Arg (R)  22	  4.7%
Asn (N)  14	  3.0%
Asp (D)  23	  4.9%
Cys (C)  10	  2.1%
Gln (Q)  16	  3.4%
Glu (E)  29	  6.1%
Gly (G)  43	  9.1%
His (H)  11	  2.3%
Ile (I)  40	  8.5%
Leu (L)  45	  9.5%
Lys (K)  36	  7.6%
Met (M)   8	  1.7%
Phe (F)  11	  2.3%
Pro (P)  25	  5.3%
Ser (S)  27	  5.7%
Thr (T)  25	  5.3%
Trp (W)   2	  0.4%
Tyr (Y)   8	  1.7%
Val (V)  44	  9.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      2271
Hydrogen    H	      3728
Nitrogen    N	       628
Oxygen      O	       667
Sulfur      S	        18

Formula: C2271H3728N628O667S18
Total number of atoms: 7312

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23545
Abs 0.1% (=1 g/l)   0.461, assuming all pairs of Cys residues form cystines


Ext. coefficient    22920
Abs 0.1% (=1 g/l)   0.449, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.70
This classifies the protein as stable.



Aliphatic index: 104.26

Grand average of hydropathicity (GRAVY): -0.008