ProtParam
User-provided sequence:
10 20 30 40 50 60
MAGIVTEPWS VAENGNPSIT AKGSSRELRL GRTAHNMSSS SLRKKSDLRV IQKVPYKGLK
70 80 90 100 110 120
DFLSNLQEVI LGTKLAILFP AIPAAIICTY CGVSQPWIFG LSLLGLTPLA ERVSFLTEQL
130 140 150 160 170 180
AFYTGPTLGG LLNATCGNAT ELIIAILALT NNKVAVVKYS LLGSILSNLL LVLGTSLFCG
190 200 210 220 230 240
GIANIRREQR FDRKQADVNF FLLLLGFLCH LLPLLVGYLK NGEASAAVLS DMQLSISRGF
250 260 270 280 290 300
SIVMLISYIA YLVFQLWTHR QLFDAQEQED EYDDDVEQET AVISFWSGFA WLVGMTLVIA
310 320 330 340 350 360
LLSEYVVATI EEASDKWNLS VSFISIILLP IVGNAAEHAG AVIFAFKNKL DISLGVALGS
370 380 390 400 410 420
ATQIGLFVVP LTIIVAWILG INMDLNFGPL ETGCLAVSII ITAFTLQDGS SHYMKGLVLL
430 440 450 460
LCYFIIAICF FVDKLPQKQN AIHLGHQAMN NVVTATGGGV FSS
References and
documentation are available.
Number of amino acids: 463
Molecular weight: 50176.79
Theoretical pI: 5.84
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 2305
Hydrogen H 3654
Nitrogen N 574
Oxygen O 641
Sulfur S 16
Formula: C
2305H
3654N
574O
641S
16
Total number of atoms: 7190
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 55390
Abs 0.1% (=1 g/l) 1.104, assuming all pairs of Cys residues form cystines
Ext. coefficient 54890
Abs 0.1% (=1 g/l) 1.094, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.65
This classifies the protein as stable.
Aliphatic index: 123.43
Grand average of hydropathicity (GRAVY): 0.601