ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGKKSPRKS TINHSTHSGK LPANIKRLIK KGESDTKSRQ SPPTLSTTRP RRFSLIYSSE 

        70         80         90        100        110        120 
SSLSDVSDSD KNKSTNPHKI KRKAKNISNN SQGKKSKLIQ RQIDNDDEGT ESSDYQAVTD 

       130        140        150        160        170        180 
GEESENEEEE SEEEEEDDDE DDDDDDDDGS DSDSDSETSS DDENIDFVKL TAQRKKRAMK 

       190        200        210        220        230        240 
ALSAMNTNSN TLYSSRENSN KNKSVKLSPK KENEEEQKEE KEKEKEEQQK QQESNKKEVN 

       250        260        270        280        290        300 
GSGTTTTQQA LSFKFKKEDD GISFGNGNEG YNEDIGEEVL DLKNKENNGN EEDKLDSKVM 

       310        320        330        340        350        360 
LGNNDELRFP NISESDESEY DIDQDAYFDV INNEDSHGEI GTDLETGEDD LPILEEEEQN 

       370        380        390        400        410        420 
IVSELQNDDE LSFDGSIHEE GSDPVEDAEN KFLQNEYNQE NGYDEEDDEE DEIMSDFDMP 

       430        440        450        460        470        480 
FYEDPKFANL YYYGDGSEPK LSLSTSLPLM LNDEKLSKLK KKEAKKREQE ERKQRRKLYK 

       490        500        510        520        530        540 
KTQKPSTRTT SNVDNDEYIF NVFFQSDDEN SGHKSKKGRH KSGKSHIEHK NKGSNLIKSN 

       550        560        570        580        590        600 
DDLEPSTHST VLNSGKYDSS DDEYDNILLD VAHMPSDDEC SESETSHDAD TDEELRALDS 

       610        620        630        640        650        660 
DSLDIGTELD DDYEDDDDDS SVTNVFIDID DLDPDSFYFH YDSDGSSSLI SSNSDKENSD 

       670        680        690        700        710        720 
GSKDCKHDLL ETVVYVDDES TDEDDNLPPP SSRSKNIGSK AKEIVSSNVV GLRPPKLGTW 

       730        740        750        760        770        780 
ETDNKPFSII DGLSTKSLYA LIQEHQQLRE QHQRAQTPDV KREGSSNGNN GDELTLNELL 

       790        800        810        820        830        840 
NMSELEDDSP SHTDDMENNY NDAINSKSTN GHAADWYEVP KVPLSAFRNK GINAYEEDEY 

       850        860        870        880        890        900 
MIPANSNRKV PIGYIGNERT RKKIDKMKEL QRKKTEKKRQ LKKKKKLLKI RKQRQKAIKE 

       910        920        930        940        950        960 
QETMNLQLGI NGHEIIGNNN SHSDINTGTD FTTNENTPMN ELPSHAPEDA SLIPHNSDLA 

       970        980        990       1000       1010       1020 
VDSNTRKNST KSVGLDEIHE ILGKDENDLL SVGDINGYDA QEGHVIEDTD ADILASLTAP 

      1030       1040       1050       1060       1070       1080 
VQFDNTLSHE NSNSMWRRRQ SMVEAAAENL RFTKNGLFSE SALADIEGIM GNDVNHSFEF 


NDVLQ 

References and documentation are available.

Number of amino acids: 1085

Molecular weight: 122491.61

Theoretical pI: 4.52

Amino acid composition: 
Ala (A)  38	  3.5%
Arg (R)  37	  3.4%
Asn (N)  92	  8.5%
Asp (D) 128	 11.8%
Cys (C)   2	  0.2%
Gln (Q)  38	  3.5%
Glu (E) 122	 11.2%
Gly (G)  55	  5.1%
His (H)  26	  2.4%
Ile (I)  52	  4.8%
Leu (L)  77	  7.1%
Lys (K)  99	  9.1%
Met (M)  17	  1.6%
Phe (F)  26	  2.4%
Pro (P)  35	  3.2%
Ser (S) 124	 11.4%
Thr (T)  53	  4.9%
Trp (W)   3	  0.3%
Tyr (Y)  27	  2.5%
Val (V)  34	  3.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 250
Total number of positively charged residues (Arg + Lys): 136

Atomic composition:

Carbon      C	      5180
Hydrogen    H	      8152
Nitrogen    N	      1480
Oxygen      O	      1920
Sulfur      S	        19

Formula: C5180H8152N1480O1920S19
Total number of atoms: 16751

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56855
Abs 0.1% (=1 g/l)   0.464, assuming all pairs of Cys residues form cystines


Ext. coefficient    56730
Abs 0.1% (=1 g/l)   0.463, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.72
This classifies the protein as unstable.



Aliphatic index: 58.96

Grand average of hydropathicity (GRAVY): -1.263