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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGLEKNADF SGKTTKSATT TSFFNRSLTI HGRTVVDSGP KSHNLNPSLN RTTSITKFYT

70 80 90 100 110 120
PVESMGTSLK GKVKNLCRLF ETSKPVKPAL AEIPQKQKSG KSLLPESRIS PFSSLNNSVI

130 140 150 160 170 180
RLPGTEDRIV VYFTSLRGIR RTYEDCYAVR MIFRGFRVWI DERDVSMDIA YRKELQIAMG

190 200 210 220 230 240
EKSVSLPQVF IMGKYVGGAD VIKSLFEIGE LAKILKEFPM RQPGFVCHCC GDIRFVPCSN

250 260 270
CSGSKKLFDE DEDRVKRCPE CNENGLIRCP DCSS


References and documentation are available.

Number of amino acids: 274 Molecular weight: 30664.40 Theoretical pI: 9.23
Amino acid composition: 
Ala (A) 10 3.6% Arg (R) 20 7.3% Asn (N) 11 4.0% Asp (D) 13 4.7% Cys (C) 11 4.0% Gln (Q) 5 1.8% Glu (E) 17 6.2% Gly (G) 19 6.9% His (H) 3 1.1% Ile (I) 17 6.2% Leu (L) 20 7.3% Lys (K) 22 8.0% Met (M) 7 2.6% Phe (F) 15 5.5% Pro (P) 15 5.5% Ser (S) 28 10.2% Thr (T) 16 5.8% Trp (W) 1 0.4% Tyr (Y) 6 2.2% Val (V) 18 6.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 30 Total number of positively charged residues (Arg + Lys): 42 Atomic composition: Carbon C 1347 Hydrogen H 2167 Nitrogen N 379 Oxygen O 401 Sulfur S 18 Formula: C1347H2167N379O401S18 Total number of atoms: 4312 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15065 Abs 0.1% (=1 g/l) 0.491, assuming all pairs of Cys residues form cystines Ext. coefficient 14440 Abs 0.1% (=1 g/l) 0.471, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 35.68 This classifies the protein as stable. Aliphatic index: 75.36 Grand average of hydropathicity (GRAVY): -0.334