ProtParam
User-provided sequence:
10 20 30 40 50 60
MAGLEKNADF SGKTTKSATT TSFFNRSLTI HGRTVVDSGP KSHNLNPSLN RTTSITKFYT
70 80 90 100 110 120
PVESMGTSLK GKVKNLCRLF ETSKPVKPAL AEIPQKQKSG KSLLPESRIS PFSSLNNSVI
130 140 150 160 170 180
RLPGTEDRIV VYFTSLRGIR RTYEDCYAVR MIFRGFRVWI DERDVSMDIA YRKELQIAMG
190 200 210 220 230 240
EKSVSLPQVF IMGKYVGGAD VIKSLFEIGE LAKILKEFPM RQPGFVCHCC GDIRFVPCSN
250 260 270
CSGSKKLFDE DEDRVKRCPE CNENGLIRCP DCSS
References and
documentation are available.
Number of amino acids: 274
Molecular weight: 30664.40
Theoretical pI: 9.23
Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1347
Hydrogen H 2167
Nitrogen N 379
Oxygen O 401
Sulfur S 18
Formula: C
1347H
2167N
379O
401S
18
Total number of atoms: 4312
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15065
Abs 0.1% (=1 g/l) 0.491, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.471, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.68
This classifies the protein as stable.
Aliphatic index: 75.36
Grand average of hydropathicity (GRAVY): -0.334