ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGLSFDNYQ RNNFLAENSH TQPKATSTGT TIVGVKFNNG VVIAADTRST QGPIVADKNC 

        70         80         90        100        110        120 
AKLHRISPKI WCAGAGTAAD TEAVTQLIGS NIELHSLYTS REPRVVSALQ MLKQHLFKYQ 

       130        140        150        160        170        180 
GHIGAYLIVA GVDPTGSHLF SIHAHGSTDV GYYLSLGSGS LAAMAVLESH WKQDLTKEEA 

       190        200        210        220        230        240 
IKLASDAIQA GIWNDLGSGS NVDVCVMEIG KDAEYLRNYL TPNVREEKQK SYKFPRGTTA 

       250        260 
VLKESIVNIC DIQEEQVDIT A 

References and documentation are available.

Number of amino acids: 261

Molecular weight: 28267.92

Theoretical pI: 6.13

Amino acid composition: 
Ala (A)  26	 10.0%
Arg (R)   8	  3.1%
Asn (N)  13	  5.0%
Asp (D)  13	  5.0%
Cys (C)   4	  1.5%
Gln (Q)  12	  4.6%
Glu (E)  14	  5.4%
Gly (G)  21	  8.0%
His (H)   9	  3.4%
Ile (I)  18	  6.9%
Leu (L)  21	  8.0%
Lys (K)  15	  5.7%
Met (M)   4	  1.5%
Phe (F)   6	  2.3%
Pro (P)   7	  2.7%
Ser (S)  21	  8.0%
Thr (T)  18	  6.9%
Trp (W)   3	  1.1%
Tyr (Y)   9	  3.4%
Val (V)  19	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1245
Hydrogen    H	      1972
Nitrogen    N	       346
Oxygen      O	       389
Sulfur      S	         8

Formula: C1245H1972N346O389S8
Total number of atoms: 3960

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30160
Abs 0.1% (=1 g/l)   1.067, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.058, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.75
This classifies the protein as stable.



Aliphatic index: 89.35

Grand average of hydropathicity (GRAVY): -0.180