ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGMASDGTQ YDPRQFDTKM NAILGEEGEE TFYTNYDEVC DSFDAMELQP DLLRGIYAYG 

        70         80         90        100        110        120 
FEKPSAIQQR GIIPFCKGLD VIQQAQSGTG KTATFCSGVL QQLDISLVQC QALVLAPTRE 

       130        140        150        160        170        180 
LAQQIEKVMR ALGDYLGVKA QACVGGTSVR EDQRVLQSGV HVVVGTPGRV FDLLRRQSLR 

       190        200        210        220        230        240 
ADAIKMFVLD EADEMLSRGF KDQIYDIFQL LPSKVQVGVF SATMPPEALE ITRKFMNKPV 

       250        260        270        280        290        300 
RILVKRDELT LEGIKQFYVN VDKEEWKLET LCDLYETLAI TQSVIFVNTR RKVDWLTDKM 

       310        320        330        340        350        360 
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINFDLPTQ 

       370        380        390        400        410 
PENYLHRIGR SGRFGRKGVA INFMTSEDER MMADIQRFYN VVVEELPSNV ADLL 

References and documentation are available.

Number of amino acids: 414

Molecular weight: 46770.52

Theoretical pI: 5.16

Amino acid composition: 
Ala (A)  25	  6.0%
Arg (R)  30	  7.2%
Asn (N)  11	  2.7%
Asp (D)  32	  7.7%
Cys (C)   6	  1.4%
Gln (Q)  28	  6.8%
Glu (E)  26	  6.3%
Gly (G)  28	  6.8%
His (H)   4	  1.0%
Ile (I)  24	  5.8%
Leu (L)  39	  9.4%
Lys (K)  18	  4.3%
Met (M)  15	  3.6%
Phe (F)  19	  4.6%
Pro (P)  13	  3.1%
Ser (S)  22	  5.3%
Thr (T)  25	  6.0%
Trp (W)   2	  0.5%
Tyr (Y)  11	  2.7%
Val (V)  36	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon      C	      2059
Hydrogen    H	      3295
Nitrogen    N	       571
Oxygen      O	       628
Sulfur      S	        21

Formula: C2059H3295N571O628S21
Total number of atoms: 6574

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27765
Abs 0.1% (=1 g/l)   0.594, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.586, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.53
This classifies the protein as unstable.



Aliphatic index: 90.60

Grand average of hydropathicity (GRAVY): -0.221