ProtParam
User-provided sequence:
10 20 30 40 50 60
MAGMASDGTQ YDPRQFDTKM NAILGEEGEE TFYTNYDEVC DSFDAMELQP DLLRGIYAYG
70 80 90 100 110 120
FEKPSAIQQR GIIPFCKGLD VIQQAQSGTG KTATFCSGVL QQLDISLVQC QALVLAPTRE
130 140 150 160 170 180
LAQQIEKVMR ALGDYLGVKA QACVGGTSVR EDQRVLQSGV HVVVGTPGRV FDLLRRQSLR
190 200 210 220 230 240
ADAIKMFVLD EADEMLSRGF KDQIYDIFQL LPSKVQVGVF SATMPPEALE ITRKFMNKPV
250 260 270 280 290 300
RILVKRDELT LEGIKQFYVN VDKEEWKLET LCDLYETLAI TQSVIFVNTR RKVDWLTDKM
310 320 330 340 350 360
RSRDHTVSAT HGDMDQNTRD IIMREFRSGS SRVLITTDLL ARGIDVQQVS LVINFDLPTQ
370 380 390 400 410
PENYLHRIGR SGRFGRKGVA INFMTSEDER MMADIQRFYN VVVEELPSNV ADLL
References and
documentation are available.
Number of amino acids: 414
Molecular weight: 46770.52
Theoretical pI: 5.16
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 2059
Hydrogen H 3295
Nitrogen N 571
Oxygen O 628
Sulfur S 21
Formula: C
2059H
3295N
571O
628S
21
Total number of atoms: 6574
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27765
Abs 0.1% (=1 g/l) 0.594, assuming all pairs of Cys residues form cystines
Ext. coefficient 27390
Abs 0.1% (=1 g/l) 0.586, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.53
This classifies the protein as unstable.
Aliphatic index: 90.60
Grand average of hydropathicity (GRAVY): -0.221