ProtParam
User-provided sequence:
10 20 30 40 50 60
MAGQQHDISH AKRAAFQVLA GGSAGFLEVC IMQPLDVVKT RIQIQATPAP NAAALGEVHY
70 80 90 100 110 120
NGVFDCFAKM YRHEGISSYW KGIMPPILAE TPKRAIKFLV FEQTKPLFQF GSPTPTPLTF
130 140 150 160 170 180
SLAGLTAGTL EAIAVNPFEV VKVAQQADRQ KKMLSTFAVA KGIIQQDGLG FSGLNKGITA
190 200 210 220 230 240
TMGRNGVFNM VYFGFYHSVK NVVPEYKESH LEFLRKVTIG FLAGTLACFV NIPFDVAKSR
250 260 270 280 290 300
IQGPQPVPGQ IKYRGTLSSM GIVYREEGFR ALYKGLVPKI MRLGPGGAIL LLVFEYSYDY
LLHNYS
References and
documentation are available.
Number of amino acids: 306
Molecular weight: 33594.20
Theoretical pI: 9.50
Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1543
Hydrogen H 2401
Nitrogen N 401
Oxygen O 415
Sulfur S 12
Formula: C
1543H
2401N
401O
415S
12
Total number of atoms: 4772
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24995
Abs 0.1% (=1 g/l) 0.744, assuming all pairs of Cys residues form cystines
Ext. coefficient 24870
Abs 0.1% (=1 g/l) 0.740, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.43
This classifies the protein as stable.
Aliphatic index: 90.52
Grand average of hydropathicity (GRAVY): 0.110