ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGTGLFAEI LDGEVYKYYA DGEWKTSSSG KSVAIMNPAT RKTQYKVQAC TQEEVNAVME 

        70         80         90        100        110        120 
LAKSAQKSWA KTPLWKRAEL LHKAAAILKD NKAPMAESLV KEIAKPAKDS VTEVVRSGDL 

       130        140        150        160        170        180 
ISYCAEEGVR ILGEGKFLLS DSFPGNDRTK YCLTSKIPLG VVLAIPPFNY PVNLAVSKIA 

       190        200        210        220        230        240 
PALIAGNSLV LKPPTQGAVS CLHMVHCFHL AGFPKGLISC ITGKGSEIGD FLTMHPAVNC 

       250        260        270        280        290        300 
ISFTGGDTGI SISKKAGMIP LQMELGGKDA CIVLDDADLD LVASNIIKGG FSYSGQRCTA 

       310        320        330        340        350        360 
VKVVLVMESV ADELVEKVKA KVAKLTVGPP EENSDITAVV SESSANFIEG LVMDAKEKGA 

       370        380        390        400        410        420 
TFCQEYKREG NLIWPLLLDN VRPDMRIAWE EPFGPVVPVL RINSVEEGIN HCNASNFGLQ 

       430        440        450        460        470        480 
GCVFTKDINK AILISDAMET GTVQINSAPA RGPDHFPFQG LKDSGIGSQG VTNSINLMTK 

       490 
VKTTVINLPT PSYSMG 

References and documentation are available.

Number of amino acids: 496

Molecular weight: 53060.33

Theoretical pI: 6.23

Amino acid composition: 
Ala (A)  45	  9.1%
Arg (R)  11	  2.2%
Asn (N)  22	  4.4%
Asp (D)  23	  4.6%
Cys (C)  12	  2.4%
Gln (Q)  12	  2.4%
Glu (E)  30	  6.0%
Gly (G)  42	  8.5%
His (H)   7	  1.4%
Ile (I)  33	  6.7%
Leu (L)  42	  8.5%
Lys (K)  39	  7.9%
Met (M)  14	  2.8%
Phe (F)  16	  3.2%
Pro (P)  27	  5.4%
Ser (S)  38	  7.7%
Thr (T)  26	  5.2%
Trp (W)   5	  1.0%
Tyr (Y)  10	  2.0%
Val (V)  42	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2359
Hydrogen    H	      3789
Nitrogen    N	       621
Oxygen      O	       711
Sulfur      S	        26

Formula: C2359H3789N621O711S26
Total number of atoms: 7506

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43150
Abs 0.1% (=1 g/l)   0.813, assuming all pairs of Cys residues form cystines


Ext. coefficient    42400
Abs 0.1% (=1 g/l)   0.799, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.49
This classifies the protein as stable.



Aliphatic index: 92.60

Grand average of hydropathicity (GRAVY): 0.025