ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGTNGKGST AAFLASILAE GGYRAGLFTS PHLTRFTERF RINGTEIAEE AAARVAGQVL 

        70         80         90        100        110        120 
ASAPEGTTFF EVVTAMALLH FVEEGVDAAI LEAGMGGGAD ATSAATGILT VIAPVALDHC 

       130        140        150        160        170        180 
EWLGETIPAI AREKAGLIRA GRPVVLSRQP AEARDVFIER GRILGAPLVC FGADFSAAWD 

       190        200        210        220        230        240 
ESSLAYDGLS RQLAGLRPGI GGRYQLANAA TALAAAEILA DNGFRLDSPA LRAGIDAARW 

       250        260        270        280        290        300 
PGRMELFSGA PRVLLDGAHN PDGARALAES LADYPRRRLI VVTGVMADKD AAGILAPVLP 

       310        320        330        340        350        360 
LAHEVVAVTP AVERGLDAGR LAELCREKGL NAVAGGTVAE GLALARQRAG EQDLILVCGS 

       370        380 
LFTVGEARAI LLSQQYEPFR G 

References and documentation are available.

Number of amino acids: 381

Molecular weight: 39542.16

Theoretical pI: 5.24

Amino acid composition: 
Ala (A)  71	 18.6%
Arg (R)  31	  8.1%
Asn (N)   6	  1.6%
Asp (D)  17	  4.5%
Cys (C)   4	  1.0%
Gln (Q)   8	  2.1%
Glu (E)  27	  7.1%
Gly (G)  44	 11.5%
His (H)   5	  1.3%
Ile (I)  18	  4.7%
Leu (L)  44	 11.5%
Lys (K)   4	  1.0%
Met (M)   5	  1.3%
Phe (F)  14	  3.7%
Pro (P)  17	  4.5%
Ser (S)  15	  3.9%
Thr (T)  18	  4.7%
Trp (W)   3	  0.8%
Tyr (Y)   5	  1.3%
Val (V)  25	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1748
Hydrogen    H	      2811
Nitrogen    N	       505
Oxygen      O	       522
Sulfur      S	         9

Formula: C1748H2811N505O522S9
Total number of atoms: 5595

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24200
Abs 0.1% (=1 g/l)   0.612, assuming all pairs of Cys residues form cystines


Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.606, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.23
This classifies the protein as stable.



Aliphatic index: 101.13

Grand average of hydropathicity (GRAVY): 0.228