ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGVLAGECS YSESGVSSHS RNSHEKQEEV SRWYFGRKEI EENSPSRLDG IDLKKETYLR 

        70         80         90        100        110        120 
KSYCTFLQDL GMRLKVPQVT IATAIIFCHR FFFRQSHAKN DRRTIATVCM FLAGKVEETP 

       130        140        150        160        170        180 
RPLKDVIFVS YEIINKKDPG ASQKIKQKEV YEQQKELILN GEKIVLSTLG FDLNVYHPYK 

       190        200        210        220        230        240 
PLVEAIKKFK VAQNALAQVA WNFVNDGLRT SLCLQFKPHH IAAGAIFLAA KFLKVKLPSD 

       250        260        270        280        290        300 
GEKVWWQEFD VTPRQLEDVS NQMLELYEQN RVPASQGSEV ESSVGGGSAQ RPGSRNAVST 

       310        320        330        340        350        360 
DEHVGSRQTS SVRSTHEQSN SDNHGGSSKG VLNQNNENGG GEAANVSVDN KEEIERETKE 

       370        380        390        400        410        420 
SSLHLESHPA HKDNVREAPH NSRPLVEGPG KDNSEREGGE LQDDGAVHKS RNVDVGDALI 

       430        440        450        460        470        480 
SQSPKDLKLL RDKVKAKREK AKKLLGERTR KKDLMDEDDL IERELEDVQL AVEDEKTKER 

       490        500        510        520        530        540 
KVQSRPKAEN SDLMGTEHGE ILDVKGEVKN TEEGEMVNNN VSPMMHSRKR KMGSPPEKQS 

       550        560        570 
EGKRRHNSEN GEESHKTSRG SSHHGDREHR RHSQENNHS 

References and documentation are available.

Number of amino acids: 579

Molecular weight: 65186.63

Theoretical pI: 6.80

Amino acid composition: 
Ala (A)  31	  5.4%
Arg (R)  38	  6.6%
Asn (N)  33	  5.7%
Asp (D)  31	  5.4%
Cys (C)   5	  0.9%
Gln (Q)  26	  4.5%
Glu (E)  62	 10.7%
Gly (G)  41	  7.1%
His (H)  24	  4.1%
Ile (I)  19	  3.3%
Leu (L)  42	  7.3%
Lys (K)  52	  9.0%
Met (M)  10	  1.7%
Phe (F)  15	  2.6%
Pro (P)  21	  3.6%
Ser (S)  55	  9.5%
Thr (T)  19	  3.3%
Trp (W)   4	  0.7%
Tyr (Y)   9	  1.6%
Val (V)  42	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      2802
Hydrogen    H	      4498
Nitrogen    N	       856
Oxygen      O	       908
Sulfur      S	        15

Formula: C2802H4498N856O908S15
Total number of atoms: 9079

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35660
Abs 0.1% (=1 g/l)   0.547, assuming all pairs of Cys residues form cystines


Ext. coefficient    35410
Abs 0.1% (=1 g/l)   0.543, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.81
This classifies the protein as unstable.



Aliphatic index: 67.48

Grand average of hydropathicity (GRAVY): -0.958