ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGVQKRKRD LEDQDDNGSE EDDIAFDIAN EIALNDSESD ANDSDSEVEA DYGPNDVQDV 

        70         80         90        100        110        120 
IEYSSDEEEG VNNKKKAENK DIKKKKNSKK EIAAFPMLEM SDDENNASGK TQTGDDEDDV 

       130        140        150        160        170        180 
NEYFSTNNLE KTKHKKGSFP SFGLSKIVLN NIKRKGFRQP TPIQRKTIPL ILQSRDIVGM 

       190        200        210        220        230        240 
ARTGSGKTAA FILPMVEKLK SHSGKIGARA VILSPSRELA MQTFNVFKDF ARGTELRSVL 

       250        260        270        280        290        300 
LTGGDSLEEQ FGMMMTNPDV IIATPGRFLH LKVEMNLDLK SVEYVVFDEA DRLFEMGFQE 

       310        320        330        340        350        360 
QLNELLASLP TTRQTLLFSA TLPNSLVDFV KAGLVNPVLV RLDAETKVSE NLEMLFLSSK 

       370        380        390        400        410        420 
NADREANLLY ILQEIIKIPL ATSEQLQKLQ NSNNEADSDS DDENDRQKKR RNFKKEKFRK 

       430        440        450        460        470        480 
QKMPAANELP SEKATILFVP TRHHVEYISQ LLRDCGYLIS YIYGTLDQHA RKRQLYNFRA 

       490        500        510        520        530        540 
GLTSILVVTD VAARGVDIPM LANVINYTLP GSSKIFVHRV GRTARAGNKG WAYSIVAENE 

       550        560        570        580        590        600 
LPYLLDLELF LGKKILLTPM YDSLVDVMKK RWIDEGKPEY QFQPPKLSYT KRLVLGSCPR 

       610        620        630        640        650        660 
LDVEGLGDLY KNLMSSNFDL QLAKKTAMKA EKLYYRTRTS ASPESLKRSK EIISSGWDAQ 

       670        680        690        700        710        720 
NAFFGKNEEK EKLDFLAKLQ NRRNKETVFE FTRNPDDEMA VFMKRRRKQL APIQRKATER 

       730        740        750        760        770        780 
RELLEKERMA GLHILSKMKF WKGDDVETGY TVSEDALKEF EDAHQLLEAQ ENENKKKKKP 

       790        800        810        820        830        840 
KSFKDPTFFL SHYAPAGDIQ DKQLQITNGF ANDAAQAAYD LNSDDKVQVH KQTATVKWDK 

       850        860        870        880        890        900 
KRKKYVNTQG IDNKKYIIGE SGQKIAASFR SGRFDDWSKA RNLKPLKVGS RETSIPSNLL 

       910        920        930        940        950        960 
EDPSQGPAAN GRTVRGKFKH KQMKAPKMPD KHRDNYYSQK KKVEKALQSG ISVKGYNNAP 

       970        980        990 
GLRSELKSTE QIRKDRIIAE KKRAKNARPS KKRKF 

References and documentation are available.

Number of amino acids: 995

Molecular weight: 113157.80

Theoretical pI: 9.29

Amino acid composition: 
Ala (A)  72	  7.2%
Arg (R)  59	  5.9%
Asn (N)  57	  5.7%
Asp (D)  71	  7.1%
Cys (C)   2	  0.2%
Gln (Q)  43	  4.3%
Glu (E)  74	  7.4%
Gly (G)  52	  5.2%
His (H)  13	  1.3%
Ile (I)  47	  4.7%
Leu (L)  93	  9.3%
Lys (K) 111	 11.2%
Met (M)  24	  2.4%
Phe (F)  42	  4.2%
Pro (P)  39	  3.9%
Ser (S)  70	  7.0%
Thr (T)  44	  4.4%
Trp (W)   6	  0.6%
Tyr (Y)  27	  2.7%
Val (V)  49	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 145
Total number of positively charged residues (Arg + Lys): 170

Atomic composition:

Carbon      C	      4994
Hydrogen    H	      8027
Nitrogen    N	      1415
Oxygen      O	      1527
Sulfur      S	        26

Formula: C4994H8027N1415O1527S26
Total number of atoms: 15989

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73355
Abs 0.1% (=1 g/l)   0.648, assuming all pairs of Cys residues form cystines


Ext. coefficient    73230
Abs 0.1% (=1 g/l)   0.647, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.92
This classifies the protein as unstable.



Aliphatic index: 76.39

Grand average of hydropathicity (GRAVY): -0.743