ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAGWQKNLQI VIRQVGKRVK DSHISTANYS STRNLESPFS QGYLQSLLRS TYSSRPLYYH 

        70         80         90        100        110        120 
LQQLGISTSR QLQAGEEPVS SPLSSPALLG SGKEEEQKII PKRQKVQAVL KSIKQSPKKV 

       130        140        150        160        170        180 
NLVAALVRGM RVEDALMQLQ VTVKRASQTV YRVIHAARAN ATHNHGLDPD RLLVAEAFVG 

       190        200        210        220        230        240 
KGLFGKKVAY HAKGRSGIIS IPRCRLTVIV RETTAEEEAE IARLKVHNFK KLNKRQRQLV 

       250        260 
PHKLIETSPI WNRRGTKGNH RSSELVPSH 

References and documentation are available.

Number of amino acids: 269

Molecular weight: 30156.84

Theoretical pI: 10.60

Amino acid composition: 
Ala (A)  20	  7.4%
Arg (R)  23	  8.6%
Asn (N)  10	  3.7%
Asp (D)   4	  1.5%
Cys (C)   1	  0.4%
Gln (Q)  18	  6.7%
Glu (E)  15	  5.6%
Gly (G)  16	  5.9%
His (H)  10	  3.7%
Ile (I)  15	  5.6%
Leu (L)  27	 10.0%
Lys (K)  22	  8.2%
Met (M)   3	  1.1%
Phe (F)   4	  1.5%
Pro (P)  12	  4.5%
Ser (S)  26	  9.7%
Thr (T)  12	  4.5%
Trp (W)   2	  0.7%
Tyr (Y)   7	  2.6%
Val (V)  22	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1330
Hydrogen    H	      2198
Nitrogen    N	       410
Oxygen      O	       381
Sulfur      S	         4

Formula: C1330H2198N410O381S4
Total number of atoms: 4323

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.711, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.711, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 63.98
This classifies the protein as unstable.



Aliphatic index: 92.04

Grand average of hydropathicity (GRAVY): -0.497