ProtParam
User-provided sequence:
10 20 30 40 50
MAHENVWFSH PRRYGKGSRQ CRVCSSHTGL IRKYGLNICR QCFREKANDI GFNKFR
References and
documentation are available.
Number of amino acids: 56
Molecular weight: 6660.66
Theoretical pI: 10.31
Total number of negatively charged residues (Asp + Glu): 3
Total number of positively charged residues (Arg + Lys): 12
Atomic composition:
Carbon C 289
Hydrogen H 451
Nitrogen N 97
Oxygen O 76
Sulfur S 5
Formula: C
289H
451N
97O
76S
5
Total number of atoms: 918
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 8730
Abs 0.1% (=1 g/l) 1.311, assuming all pairs of Cys residues form cystines
Ext. coefficient 8480
Abs 0.1% (=1 g/l) 1.273, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.74
This classifies the protein as stable.
Aliphatic index: 48.75
Grand average of hydropathicity (GRAVY): -0.848