ProtParam
User-provided sequence:
10 20 30 40 50 60
MAHRAFIQVI ITGTQVFGKA FAEAYRQAAS QSVKQGATNA SRRGTGKGEY GGITLDESCK
70 80 90 100 110 120
ILNIEESKGD LNMDKINNRF NYLFEVNDKE KGGSFYLQSK VYRAAERLKW ELAQREKNAK
130 140
AKAGDASTAK PPPNSTNSSG ADNSASSNQ
References and
documentation are available.
Number of amino acids: 149
Molecular weight: 16216.00
Theoretical pI: 9.46
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 700
Hydrogen H 1114
Nitrogen N 210
Oxygen O 228
Sulfur S 3
Formula: C
700H
1114N
210O
228S
3
Total number of atoms: 2255
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.799, assuming all pairs of Cys residues form cystines
Ext. coefficient 12950
Abs 0.1% (=1 g/l) 0.799, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.77
This classifies the protein as stable.
Aliphatic index: 59.13
Grand average of hydropathicity (GRAVY): -0.785