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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAHRAFIQVI ITGTQVFGKA FAEAYRQAAS QSVKQGATNA SRRGTGKGEY GGITLDESCK

70 80 90 100 110 120
ILNIEESKGD LNMDKINNRF NYLFEVNDKE KGGSFYLQSK VYRAAERLKW ELAQREKNAK

130 140
AKAGDASTAK PPPNSTNSSG ADNSASSNQ


References and documentation are available.

Number of amino acids: 149 Molecular weight: 16216.00 Theoretical pI: 9.46
Amino acid composition: 
Ala (A) 19 12.8% Arg (R) 8 5.4% Asn (N) 12 8.1% Asp (D) 6 4.0% Cys (C) 1 0.7% Gln (Q) 8 5.4% Glu (E) 10 6.7% Gly (G) 13 8.7% His (H) 1 0.7% Ile (I) 7 4.7% Leu (L) 7 4.7% Lys (K) 14 9.4% Met (M) 2 1.3% Phe (F) 6 4.0% Pro (P) 3 2.0% Ser (S) 14 9.4% Thr (T) 7 4.7% Trp (W) 1 0.7% Tyr (Y) 5 3.4% Val (V) 5 3.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 16 Total number of positively charged residues (Arg + Lys): 22 Atomic composition: Carbon C 700 Hydrogen H 1114 Nitrogen N 210 Oxygen O 228 Sulfur S 3 Formula: C700H1114N210O228S3 Total number of atoms: 2255 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 12950 Abs 0.1% (=1 g/l) 0.799, assuming all pairs of Cys residues form cystines Ext. coefficient 12950 Abs 0.1% (=1 g/l) 0.799, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 30.77 This classifies the protein as stable. Aliphatic index: 59.13 Grand average of hydropathicity (GRAVY): -0.785