ProtParam
User-provided sequence:
10 20 30 40 50 60
MAHYHDDYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT LRSKKDYLHG
70 80 90 100 110 120
DNSDIIPTDT IKNTVHVLAK FKGIKSIETF AMNICEHFLS SFSHVTRAHV YVEEVPWKRF
130 140 150 160 170 180
EKNGVKHVHA FIHTPTGTHF CDVEQVRNGP PIIHSGIKDL KVLKTTQSGF EGFIKDQFTT
190 200 210 220 230 240
LPEVKDRCFA TQVYCKWRYQ NRDVDFEATW GAVRDIVLKK FAGPYDRGEY SPSVQKTLYD
250 260 270 280 290 300
IQVLTLSQLP EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVRRKLP
SRL
References and
documentation are available.
Number of amino acids: 303
Molecular weight: 34933.97
Theoretical pI: 8.19
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1576
Hydrogen H 2450
Nitrogen N 426
Oxygen O 453
Sulfur S 10
Formula: C
1576H
2450N
426O
453S
10
Total number of atoms: 4915
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36120
Abs 0.1% (=1 g/l) 1.034, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 1.027, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.30
This classifies the protein as stable.
Aliphatic index: 82.24
Grand average of hydropathicity (GRAVY): -0.450