ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAHYHDDYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT LRSKKDYLHG 

        70         80         90        100        110        120 
DNSDIIPTDT IKNTVHVLAK FKGIKSIETF AMNICEHFLS SFSHVTRAHV YVEEVPWKRF 

       130        140        150        160        170        180 
EKNGVKHVHA FIHTPTGTHF CDVEQVRNGP PIIHSGIKDL KVLKTTQSGF EGFIKDQFTT 

       190        200        210        220        230        240 
LPEVKDRCFA TQVYCKWRYQ NRDVDFEATW GAVRDIVLKK FAGPYDRGEY SPSVQKTLYD 

       250        260        270        280        290        300 
IQVLTLSQLP EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVRRKLP 


SRL 

References and documentation are available.

Number of amino acids: 303

Molecular weight: 34933.97

Theoretical pI: 8.19

Amino acid composition: 
Ala (A)  10	  3.3%
Arg (R)  14	  4.6%
Asn (N)  11	  3.6%
Asp (D)  21	  6.9%
Cys (C)   4	  1.3%
Gln (Q)  10	  3.3%
Glu (E)  18	  5.9%
Gly (G)  18	  5.9%
His (H)  15	  5.0%
Ile (I)  21	  6.9%
Leu (L)  21	  6.9%
Lys (K)  27	  8.9%
Met (M)   6	  2.0%
Phe (F)  15	  5.0%
Pro (P)  13	  4.3%
Ser (S)  16	  5.3%
Thr (T)  21	  6.9%
Trp (W)   3	  1.0%
Tyr (Y)  13	  4.3%
Val (V)  26	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1576
Hydrogen    H	      2450
Nitrogen    N	       426
Oxygen      O	       453
Sulfur      S	        10

Formula: C1576H2450N426O453S10
Total number of atoms: 4915

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36120
Abs 0.1% (=1 g/l)   1.034, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   1.027, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.30
This classifies the protein as stable.



Aliphatic index: 82.24

Grand average of hydropathicity (GRAVY): -0.450