ProtParam
User-provided sequence:
10 20 30 40 50 60
MAIDYSKWDK IELSDDSDVE VHPNVDKKSF IKWKQQSIHE QRFKRNQDIK NLETQVDMYS
70 80 90 100 110 120
HLNKRVDRIL SNLPESSLTD LPAVTKFLNA NFDKMEKSKG ENVDPEIATY NEMVEDLFEQ
130 140 150 160 170 180
LAKDLDKEGK DSKSPSLIRD AILKHRAKID SVTVEAKKKL DELYKEKNAH ISSEDIHTGF
190 200 210 220 230 240
DSSFMNKQKG GAKPLEATPS EALSSAAESN ILNKLAKSSV PQTFIDFKDD PMKLAKETEE
250 260 270 280 290 300
FGKISINEYS KSQKFLLEHL PIISEQQKDA LMMKAFEYQL HGDDKMTLQV IHQSELMAYI
310 320 330 340 350 360
KEIYDMKKIP YLNPMELSNV INMFFEKVIF NKDKPMGKES FLRSVQEKFL HIQKRSKILQ
370 380 390 400 410 420
QEEMDESNAE GVETIQLKSL DDSTELEVNL PDFNSKDPEE MKKVKVFKTL IPEKMQEAIM
430 440 450 460 470 480
TKNLDNINKV FEDIPIEEAE KLLEVFNDID IIGIKAILEN EKDFQSLKDQ YEQDHEDATM
490 500
ENLSLNDRDG GGDNHEEVKH TADTVD
References and
documentation are available.
Number of amino acids: 506
Molecular weight: 58385.02
Theoretical pI: 4.93
Total number of negatively charged residues (Asp + Glu): 102
Total number of positively charged residues (Arg + Lys): 71
Atomic composition:
Carbon C 2575
Hydrogen H 4097
Nitrogen N 675
Oxygen O 829
Sulfur S 19
Formula: C
2575H
4097N
675O
829S
19
Total number of atoms: 8195
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 0.444
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.44
This classifies the protein as unstable.
Aliphatic index: 80.55
Grand average of hydropathicity (GRAVY): -0.751