ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAIIFALVAR GTVVLSEFSA TSTNASSISK QILEKLPGND SDSHMSYSQD RYIFHVKRTD 

        70         80         90        100        110        120 
GLTVLCMADE TAGRNIPFAF LDDIHQRFVK TYGRAIHSAQ AYSMNDEFSR VLSQQMEFYS 

       130        140        150        160        170        180 
NDPNADRMSR IKGEMSQVRN VMIENIDKVL DRGERLELLV DKTENMQGNT FRFRKQARRY 

       190        200        210        220 
RTIMWWRNVK LTIALILVLA LVVYIAMAFV CHGPSLPSCF K 

References and documentation are available.

Number of amino acids: 221

Molecular weight: 25320.19

Theoretical pI: 9.17

Amino acid composition: 
Ala (A)  17	  7.7%
Arg (R)  18	  8.1%
Asn (N)  11	  5.0%
Asp (D)  13	  5.9%
Cys (C)   3	  1.4%
Gln (Q)   9	  4.1%
Glu (E)  10	  4.5%
Gly (G)   9	  4.1%
His (H)   5	  2.3%
Ile (I)  15	  6.8%
Leu (L)  18	  8.1%
Lys (K)  10	  4.5%
Met (M)  11	  5.0%
Phe (F)  12	  5.4%
Pro (P)   5	  2.3%
Ser (S)  19	  8.6%
Thr (T)  11	  5.0%
Trp (W)   2	  0.9%
Tyr (Y)   7	  3.2%
Val (V)  16	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1119
Hydrogen    H	      1777
Nitrogen    N	       317
Oxygen      O	       325
Sulfur      S	        14

Formula: C1119H1777N317O325S14
Total number of atoms: 3552

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21555
Abs 0.1% (=1 g/l)   0.851, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.846, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.22
This classifies the protein as unstable.



Aliphatic index: 86.92

Grand average of hydropathicity (GRAVY): -0.164