ProtParam
User-provided sequence:
10 20 30 40 50 60
MAIIPLAQLN ELTISSSSSS FLTKSISSHS LHSSCICASS RISQFRGGFS KRRSDSTRSK
70 80 90 100 110 120
SMRLRCSFSP MESARIKVIG VGGGGNNAVN RMISSGLQSV DFYAINTDSQ ALLQSSAENP
130 140 150 160 170 180
LQIGELLTRG LGTGGNPLLG EQAAEESKDA IANALKGSDL VFITAGMGGG TGSGAAPVVA
190 200 210 220 230 240
QISKDAGYLT VGVVTYPFSF EGRKRSLQAL EAIEKLQKNV DTLIVIPNDR LLDIADEQTP
250 260 270 280 290 300
LQDAFLLADD VLRQGVQGIS DIITIPGLVN VDFADVKAVM KDSGTAMLGV GVSSSKNRAE
310 320 330 340 350 360
EAAEQATLAP LIGSSIQSAT GVVYNITGGK DITLQEVNRV SQVVTSLADP SANIIFGAVV
370 380 390 400 410 420
DDRYTGEIHV TIIATGFSQS FQKTLLTDPR AAKLLDKMGS SGQQENKGMS LPHQKQSPST
430
ISTKSSSPRR LFF
References and
documentation are available.
Number of amino acids: 433
Molecular weight: 45564.62
Theoretical pI: 6.92
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1980
Hydrogen H 3243
Nitrogen N 561
Oxygen O 642
Sulfur S 12
Formula: C
1980H
3243N
561O
642S
12
Total number of atoms: 6438
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 7575
Abs 0.1% (=1 g/l) 0.166, assuming all pairs of Cys residues form cystines
Ext. coefficient 7450
Abs 0.1% (=1 g/l) 0.164, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.34
This classifies the protein as unstable.
Aliphatic index: 93.05
Grand average of hydropathicity (GRAVY): -0.042