ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAIIPLAQLN ELTISSSSSS FLTKSISSHS LHSSCICASS RISQFRGGFS KRRSDSTRSK 

        70         80         90        100        110        120 
SMRLRCSFSP MESARIKVIG VGGGGNNAVN RMISSGLQSV DFYAINTDSQ ALLQSSAENP 

       130        140        150        160        170        180 
LQIGELLTRG LGTGGNPLLG EQAAEESKDA IANALKGSDL VFITAGMGGG TGSGAAPVVA 

       190        200        210        220        230        240 
QISKDAGYLT VGVVTYPFSF EGRKRSLQAL EAIEKLQKNV DTLIVIPNDR LLDIADEQTP 

       250        260        270        280        290        300 
LQDAFLLADD VLRQGVQGIS DIITIPGLVN VDFADVKAVM KDSGTAMLGV GVSSSKNRAE 

       310        320        330        340        350        360 
EAAEQATLAP LIGSSIQSAT GVVYNITGGK DITLQEVNRV SQVVTSLADP SANIIFGAVV 

       370        380        390        400        410        420 
DDRYTGEIHV TIIATGFSQS FQKTLLTDPR AAKLLDKMGS SGQQENKGMS LPHQKQSPST 

       430 
ISTKSSSPRR LFF 

References and documentation are available.

Number of amino acids: 433

Molecular weight: 45564.62

Theoretical pI: 6.92

Amino acid composition: 
Ala (A)  38	  8.8%
Arg (R)  20	  4.6%
Asn (N)  16	  3.7%
Asp (D)  23	  5.3%
Cys (C)   3	  0.7%
Gln (Q)  24	  5.5%
Glu (E)  17	  3.9%
Gly (G)  40	  9.2%
His (H)   4	  0.9%
Ile (I)  32	  7.4%
Leu (L)  40	  9.2%
Lys (K)  20	  4.6%
Met (M)   9	  2.1%
Phe (F)  15	  3.5%
Pro (P)  15	  3.5%
Ser (S)  57	 13.2%
Thr (T)  26	  6.0%
Trp (W)   0	  0.0%
Tyr (Y)   5	  1.2%
Val (V)  29	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1980
Hydrogen    H	      3243
Nitrogen    N	       561
Oxygen      O	       642
Sulfur      S	        12

Formula: C1980H3243N561O642S12
Total number of atoms: 6438

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7575
Abs 0.1% (=1 g/l)   0.166, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.164, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.34
This classifies the protein as unstable.



Aliphatic index: 93.05

Grand average of hydropathicity (GRAVY): -0.042