ProtParam
User-provided sequence:
10 20 30 40 50 60
MAIKPRTKGK TYSSRSVGSQ WFNRLGFKQN KYGTCKFLSI ITAFVFILYF FSNRFYPISR
70 80 90 100 110 120
SAGASYSPSH GLYINEIPAS SRLIYPHVEH VPVLKQMTVR GLYITRLEVD GSKRLILKPE
130 140 150 160 170 180
ENALTDEEKK KTTDQILLVK HSFLDHGKLV YRKSNDAPEV VVVTLIDFEN YELETIIQIV
190 200 210 220 230 240
QNRVDYAQKH QYGVYIRWIQ EFLPVLENQN LAESYEFIKP LVIRAAMHAF PTAKYIHFVD
250 260 270 280 290 300
QDALLMNLDL SLQKYLLDPK IMDLALLKNV PVVANSNIKT YNHFEYSSAK IIIPHDAGGN
310 320 330 340 350 360
IDASSFVIAN DFYGKALIDY LNDPLLRNFP WDNTGDKLSA AIGHILQWHP TLLGKTAIVI
370 380 390 400 410 420
PKVLASQYDA SLDQEGESGN GASNGDVYHY NEGDLAASFK GCRSRGTCAS EIGHMYQKIK
KS
References and
documentation are available.
Number of amino acids: 422
Molecular weight: 47800.78
Theoretical pI: 8.61
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 2178
Hydrogen H 3378
Nitrogen N 572
Oxygen O 621
Sulfur S 9
Formula: C
2178H
3378N
572O
621S
9
Total number of atoms: 6758
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57885
Abs 0.1% (=1 g/l) 1.211, assuming all pairs of Cys residues form cystines
Ext. coefficient 57760
Abs 0.1% (=1 g/l) 1.208, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.73
This classifies the protein as stable.
Aliphatic index: 95.21
Grand average of hydropathicity (GRAVY): -0.207