ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAIKPRTKGK TYSSRSVGSQ WFNRLGFKQN KYGTCKFLSI ITAFVFILYF FSNRFYPISR 

        70         80         90        100        110        120 
SAGASYSPSH GLYINEIPAS SRLIYPHVEH VPVLKQMTVR GLYITRLEVD GSKRLILKPE 

       130        140        150        160        170        180 
ENALTDEEKK KTTDQILLVK HSFLDHGKLV YRKSNDAPEV VVVTLIDFEN YELETIIQIV 

       190        200        210        220        230        240 
QNRVDYAQKH QYGVYIRWIQ EFLPVLENQN LAESYEFIKP LVIRAAMHAF PTAKYIHFVD 

       250        260        270        280        290        300 
QDALLMNLDL SLQKYLLDPK IMDLALLKNV PVVANSNIKT YNHFEYSSAK IIIPHDAGGN 

       310        320        330        340        350        360 
IDASSFVIAN DFYGKALIDY LNDPLLRNFP WDNTGDKLSA AIGHILQWHP TLLGKTAIVI 

       370        380        390        400        410        420 
PKVLASQYDA SLDQEGESGN GASNGDVYHY NEGDLAASFK GCRSRGTCAS EIGHMYQKIK 


KS 

References and documentation are available.

Number of amino acids: 422

Molecular weight: 47800.78

Theoretical pI: 8.61

Amino acid composition: 
Ala (A)  30	  7.1%
Arg (R)  16	  3.8%
Asn (N)  23	  5.5%
Asp (D)  23	  5.5%
Cys (C)   3	  0.7%
Gln (Q)  16	  3.8%
Glu (E)  20	  4.7%
Gly (G)  24	  5.7%
His (H)  14	  3.3%
Ile (I)  34	  8.1%
Leu (L)  42	 10.0%
Lys (K)  31	  7.3%
Met (M)   6	  1.4%
Phe (F)  19	  4.5%
Pro (P)  18	  4.3%
Ser (S)  32	  7.6%
Thr (T)  17	  4.0%
Trp (W)   4	  0.9%
Tyr (Y)  24	  5.7%
Val (V)  26	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 47

Atomic composition:

Carbon      C	      2178
Hydrogen    H	      3378
Nitrogen    N	       572
Oxygen      O	       621
Sulfur      S	         9

Formula: C2178H3378N572O621S9
Total number of atoms: 6758

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57885
Abs 0.1% (=1 g/l)   1.211, assuming all pairs of Cys residues form cystines


Ext. coefficient    57760
Abs 0.1% (=1 g/l)   1.208, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.73
This classifies the protein as stable.



Aliphatic index: 95.21

Grand average of hydropathicity (GRAVY): -0.207