ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAINNENELE YKLIKKNKNP KISNSKKKNS TRPALQDKTN QTLPIHQNQA FSNILPSDFS 

        70         80         90        100        110        120 
IIKTPETKTA DDFPVNGYEG LNILKFDLEL FYKLKPVATS TPKSCMRTGS NLFLNETVKH 

       130        140        150        160        170 
VPDERLVSNI KNTQTKDSIT RDSAYYHRKT MTESIIKTLA AFDAEVDEII LF 

References and documentation are available.

Number of amino acids: 172

Molecular weight: 19612.37

Theoretical pI: 9.04

Amino acid composition: 
Ala (A)   9	  5.2%
Arg (R)   5	  2.9%
Asn (N)  16	  9.3%
Asp (D)  10	  5.8%
Cys (C)   1	  0.6%
Gln (Q)   5	  2.9%
Glu (E)  11	  6.4%
Gly (G)   3	  1.7%
His (H)   3	  1.7%
Ile (I)  14	  8.1%
Leu (L)  15	  8.7%
Lys (K)  20	 11.6%
Met (M)   3	  1.7%
Phe (F)   8	  4.7%
Pro (P)   9	  5.2%
Ser (S)  13	  7.6%
Thr (T)  16	  9.3%
Trp (W)   0	  0.0%
Tyr (Y)   5	  2.9%
Val (V)   6	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	       872
Hydrogen    H	      1402
Nitrogen    N	       234
Oxygen      O	       270
Sulfur      S	         4

Formula: C872H1402N234O270S4
Total number of atoms: 2782

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.380, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.380, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.06
This classifies the protein as stable.



Aliphatic index: 81.10

Grand average of hydropathicity (GRAVY): -0.633