ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAIQKVSNKD LSRKDKRRFN IESKVNKIYQ NFYSERDNQY KDRLTALQTD LTSLHQGDNG 

        70         80         90        100        110        120 
QYARQVRDLE EERDLELVRL RLFEEYRVSR SGIEFQEDIE KAKAEHEKLI KLCKERLYSS 

       130        140        150        160        170        180 
IEQKIKKLQE ERLLMDVANV HSYAMNYSRP QYQKNTRSHT VSGWDSSSNE YGRDTANESA 

       190        200        210        220        230        240 
TDTGAGNDRR TLRRRNASKD TRGNNNNQDE SDFQTGNGSG SNGHGSRQGS QFPHFNNLTY 

       250        260        270        280        290        300 
KSGMNSDSDF LQGINEGTDL YAFLFGEKNP KDNANGNEKK KNRGAQRYST KTAPPLQSLK 

       310        320 
PDEVTEDISL IRELTGQPPA PFRLRSD 

References and documentation are available.

Number of amino acids: 327

Molecular weight: 37625.28

Theoretical pI: 8.86

Amino acid composition: 
Ala (A)  16	  4.9%
Arg (R)  29	  8.9%
Asn (N)  28	  8.6%
Asp (D)  24	  7.3%
Cys (C)   1	  0.3%
Gln (Q)  20	  6.1%
Glu (E)  26	  8.0%
Gly (G)  21	  6.4%
His (H)   6	  1.8%
Ile (I)  11	  3.4%
Leu (L)  26	  8.0%
Lys (K)  25	  7.6%
Met (M)   4	  1.2%
Phe (F)  11	  3.4%
Pro (P)   9	  2.8%
Ser (S)  30	  9.2%
Thr (T)  17	  5.2%
Trp (W)   1	  0.3%
Tyr (Y)  13	  4.0%
Val (V)   9	  2.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      1608
Hydrogen    H	      2552
Nitrogen    N	       500
Oxygen      O	       536
Sulfur      S	         5

Formula: C1608H2552N500O536S5
Total number of atoms: 5201

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.661, assuming all pairs of Cys residues form cystines


Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.661, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.89
This classifies the protein as unstable.



Aliphatic index: 57.00

Grand average of hydropathicity (GRAVY): -1.257