ProtParam
User-provided sequence:
10 20 30 40 50 60
MAIREIKDVE RGEIVNKVED LGKPFLTHED DEKESENNES YLMVLFSTFV AVCGSFEFGS
70 80 90 100 110 120
CVGYSAPTQS SIRQDLNLSL AEFSMFGSIL TIGAMLGAVM SGKISDFSGR KGAMRTSACF
130 140 150 160 170 180
CITGWLAVFF TKGALLLDVG RFFTGYGIGV FSYVVPVYIA EISPKNLRGG LTTLNQLMIV
190 200 210 220 230 240
IGSSVSFLIG SLISWKTLAL TGLAPCIVLL FGLCFIPESP RWLAKAGHEK EFRVALQKLR
250 260 270 280 290 300
GKDADITNEA DGIQVSIQAL EILPKARIQD LVSKKYGRSV IIGVSLMVFQ QFVGINGIGF
310 320 330 340 350 360
YASETFVKAG FTSGKLGTIA IACVQVPITV LGTILIDKSG RRPLIMISAG GIFLGCILTG
370 380 390 400 410 420
TSFLLKGQSL LLEWVPSLAV GGVLIYVAAF SIGMGPVPWV IMSEIFPINV KGIAGSLVVL
430 440 450 460 470 480
VNWSGAWAVS YTFNFLMSWS SPGTFYLYSA FAAATIIFVA KMVPETKGKT LEEIQACIRR
ET
References and
documentation are available.
Number of amino acids: 482
Molecular weight: 51992.12
Theoretical pI: 8.47
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 2390
Hydrogen H 3773
Nitrogen N 589
Oxygen O 658
Sulfur S 22
Formula: C
2390H
3773N
589O
658S
22
Total number of atoms: 7432
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 60890
Abs 0.1% (=1 g/l) 1.171, assuming all pairs of Cys residues form cystines
Ext. coefficient 60390
Abs 0.1% (=1 g/l) 1.162, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.55
This classifies the protein as stable.
Aliphatic index: 109.21
Grand average of hydropathicity (GRAVY): 0.560