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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAIRKEEESR EEQSNSFLLD ALYCEEEKWD DEGEEVEENS SLSSSSSPFV VLQQDLFWED

70 80 90 100 110 120
EDLVTLFSKE EEQGLSCLDD VYLSTDRKEA VGWILRVNAH YGFSTLAAVL AITYLDKFIC

130 140 150 160 170 180
SYSLQRDKPW MLQLVSVACL SLAAKVEETQ VPLLLDFQVE ETKYVFEAKT IQRMELLILS

190 200 210 220 230 240
TLEWKMHLIT PISFVDHIIR RLGLKNNAHW DFLNKCHRLL LSVISDSRFV GYLPSVVAAA

250 260 270 280 290 300
TMMRIIEQVD PFDPLSYQTN LLGVLNLTKE KVKTCYDLIL QLPVDRIGLQ IQIQSSKKRK

310 320 330 340 350 360
SHDSSSSLNS PSCVIDANPF NSDESSNDSW SASSCNPPTS SSSPQQQPPL KKMRGAEENE

370
KKKPILHLPW AIVATP


References and documentation are available.

Number of amino acids: 376 Molecular weight: 42701.61 Theoretical pI: 4.89
Amino acid composition: 
Ala (A) 20 5.3% Arg (R) 14 3.7% Asn (N) 14 3.7% Asp (D) 23 6.1% Cys (C) 8 2.1% Gln (Q) 18 4.8% Glu (E) 32 8.5% Gly (G) 9 2.4% His (H) 7 1.9% Ile (I) 20 5.3% Leu (L) 48 12.8% Lys (K) 23 6.1% Met (M) 7 1.9% Phe (F) 13 3.5% Pro (P) 18 4.8% Ser (S) 45 12.0% Thr (T) 15 4.0% Trp (W) 8 2.1% Tyr (Y) 9 2.4% Val (V) 25 6.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 55 Total number of positively charged residues (Arg + Lys): 37 Atomic composition: Carbon C 1903 Hydrogen H 2999 Nitrogen N 495 Oxygen O 588 Sulfur S 15 Formula: C1903H2999N495O588S15 Total number of atoms: 6000 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 57910 Abs 0.1% (=1 g/l) 1.356, assuming all pairs of Cys residues form cystines Ext. coefficient 57410 Abs 0.1% (=1 g/l) 1.344, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 63.79 This classifies the protein as unstable. Aliphatic index: 95.13 Grand average of hydropathicity (GRAVY): -0.251