ProtParam
User-provided sequence:
10 20 30 40 50 60
MAIRKEEESR EEQSNSFLLD ALYCEEEKWD DEGEEVEENS SLSSSSSPFV VLQQDLFWED
70 80 90 100 110 120
EDLVTLFSKE EEQGLSCLDD VYLSTDRKEA VGWILRVNAH YGFSTLAAVL AITYLDKFIC
130 140 150 160 170 180
SYSLQRDKPW MLQLVSVACL SLAAKVEETQ VPLLLDFQVE ETKYVFEAKT IQRMELLILS
190 200 210 220 230 240
TLEWKMHLIT PISFVDHIIR RLGLKNNAHW DFLNKCHRLL LSVISDSRFV GYLPSVVAAA
250 260 270 280 290 300
TMMRIIEQVD PFDPLSYQTN LLGVLNLTKE KVKTCYDLIL QLPVDRIGLQ IQIQSSKKRK
310 320 330 340 350 360
SHDSSSSLNS PSCVIDANPF NSDESSNDSW SASSCNPPTS SSSPQQQPPL KKMRGAEENE
370
KKKPILHLPW AIVATP
References and
documentation are available.
Number of amino acids: 376
Molecular weight: 42701.61
Theoretical pI: 4.89
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1903
Hydrogen H 2999
Nitrogen N 495
Oxygen O 588
Sulfur S 15
Formula: C
1903H
2999N
495O
588S
15
Total number of atoms: 6000
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57910
Abs 0.1% (=1 g/l) 1.356, assuming all pairs of Cys residues form cystines
Ext. coefficient 57410
Abs 0.1% (=1 g/l) 1.344, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 63.79
This classifies the protein as unstable.
Aliphatic index: 95.13
Grand average of hydropathicity (GRAVY): -0.251