ProtParam
User-provided sequence:
10 20 30 40 50 60
MAIVNSWLIC LVSIFSFVVR VEAATFCNAT QACPEDKPCC SQYGECGTGQ YCLNNCDVRY
70 80 90 100 110 120
SFSHDSCMPV PICKSSSTKF KDYSSKLGNA NTFLGNVSEA DWLYTGDVLD YDDEESLILA
130 140 150 160 170 180
MPKNSGGTVL SSTRAVWYGK VSARIKTSHL AGVVTGFILY SGAGDELDYE FVGADLETAQ
190 200 210 220 230 240
TNFYWESVLN YTNSANISTT DTFENYHTYE LDWHEDYVTW SIDGVVGRTL YKNETYNATT
250 260 270 280 290 300
QKYQYPQTPS KVDISIWPGG NSTNAPGTIA WSGGEINWDA SDISNPGYYY AIVNEVNITC
310 320 330 340 350 360
YDPPSDTKKN GTSAYVYTSS SEFLAKDIAI TDDEVMMDSD EGSGLDPHKG ATTSSTQKSS
370 380 390 400 410 420
SSTATSSSKT SSDHSSSTKK SSKTSSTASS SSSSSSSSSS SSSTATKNGD KVVSSVSSSV
430 440 450 460
TSQTQTTSSV SGSASSSTSS MSGNNAGANV AANWRLTVLC VILGYVL
References and
documentation are available.
Number of amino acids: 467
Molecular weight: 49906.37
Theoretical pI: 4.60
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 2159
Hydrogen H 3300
Nitrogen N 566
Oxygen O 759
Sulfur S 18
Formula: C
2159H
3300N
566O
759S
18
Total number of atoms: 6802
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 93000
Abs 0.1% (=1 g/l) 1.863, assuming all pairs of Cys residues form cystines
Ext. coefficient 92250
Abs 0.1% (=1 g/l) 1.848, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.09
This classifies the protein as stable.
Aliphatic index: 62.42
Grand average of hydropathicity (GRAVY): -0.352