ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAKDNLTNLL SQLNIQLSQD EHSQVEQTCV KLLDSGCENP ADVFRRCLVA VIQQDKYQKA 

        70         80         90        100        110        120 
LHYLKKFKHI DDKYGRKFAL EKLYIFYKLN MPDEFNTLYT AIITDDLDTV LKKDIESLRG 

       130        140        150        160        170        180 
ILHVRAQYCY KNGLYQEAFK IYQHLASHNE KDQDSQIELS CNERVPLSVA TELMNRSPLV 

       190        200        210        220        230        240 
TPMDESSYDL LFNESFIMAS VGKYDKAIEL LEKALQGATN EGYQNDINTI KLQLSFVLQM 

       250        260        270        280        290        300 
VGKTAQSKEI LKGLLQELKA DSPFSLICQN NLNAFVDFSK YNTNFNLLLR ELNVEKLNTF 

       310        320        330        340        350        360 
NLQTFTHEQW SNIQRNVLFL RLFNNVKIHS QESLLSRTFD KYSKLVDNVT LESYKTQAKK 

       370        380        390        400        410        420 
LYHHTTKTIL SGTDGSTIGI LLLTIQLLII EKEWENAIRI GELFLNESWK SSFEKFNDSQ 

       430        440        450        460        470        480 
AIVCYILFEL YKIKGRNNSK SVLLKKLGSV RVQLSGKIQE NIPFWKHVGF ELLSMGNAKE 

       490        500        510        520        530        540 
SKALLREISN FSKGDADVLV DRVVSSDSLD IAQGIDLVRD IDIDKLIQLG VKPLESSAKR 

       550        560        570        580        590        600 
SKNTAVSKVQ KRKVLELKKK RKIKRLEKFL QGRDTSKLPD PERWLPLRDR STYRPKKKQQ 

       610        620        630        640 
GAKQTQGGAM NKKSEQALDI SKKGKPTVNK KPKNKKKGRK 

References and documentation are available.

Number of amino acids: 640

Molecular weight: 73540.85

Theoretical pI: 9.44

Amino acid composition: 
Ala (A)  29	  4.5%
Arg (R)  27	  4.2%
Asn (N)  40	  6.2%
Asp (D)  37	  5.8%
Cys (C)   7	  1.1%
Gln (Q)  38	  5.9%
Glu (E)  39	  6.1%
Gly (G)  27	  4.2%
His (H)  11	  1.7%
Ile (I)  39	  6.1%
Leu (L)  84	 13.1%
Lys (K)  76	 11.9%
Met (M)   8	  1.2%
Phe (F)  26	  4.1%
Pro (P)  14	  2.2%
Ser (S)  50	  7.8%
Thr (T)  29	  4.5%
Trp (W)   5	  0.8%
Tyr (Y)  20	  3.1%
Val (V)  34	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 103

Atomic composition:

Carbon      C	      3292
Hydrogen    H	      5324
Nitrogen    N	       902
Oxygen      O	       970
Sulfur      S	        15

Formula: C3292H5324N902O970S15
Total number of atoms: 10503

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57675
Abs 0.1% (=1 g/l)   0.784, assuming all pairs of Cys residues form cystines


Ext. coefficient    57300
Abs 0.1% (=1 g/l)   0.779, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.22
This classifies the protein as stable.



Aliphatic index: 94.89

Grand average of hydropathicity (GRAVY): -0.501